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Titolo:
Inhibition of HIV-1 replication by a new type of circular dumbbell RNA/DNAchimeric oligonucleotides
Autore:
Park, WS; Miyano-Kurosaki, N; Abe, T; Takai, K; Yamamoto, N; Takaku, H;
Indirizzi:
Chiba Inst Technol, Dept Ind Chem, Chiba 2750016, Japan Chiba Inst Technol Chiba Japan 2750016 pt Ind Chem, Chiba 2750016, Japan Chiba Inst Technol, High Technol Res Ctr, Chiba 2750016, Japan Chiba Inst Technol Chiba Japan 2750016 nol Res Ctr, Chiba 2750016, Japan Tokyo Med & Dent Univ, Sch Med, Dept Microbiol, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 270, anno: 2000,
pagine: 953 - 960
SICI:
0006-291X(20000421)270:3<953:IOHRBA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN IMMUNODEFICIENCY VIRUS; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE; ANTISENSE OLIGONUCLEOTIDES; EXONUCLEASE DEGRADATION; REVERSE-TRANSCRIPTASE; BINDING; HAIRPIN; CELLS; DNA; 3'-EXONUCLEASE;
Keywords:
circular dumbbell RNA/DNA chimeric; oligonucleotide; antisense phosphodiester oligonucleotide; RNase H; exonuclease resistance; cellular uptake; inhibition of HIV-1 replication;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Takaku, H Chiba Inst Technol, Dept Ind Chem, 2-17-1 Tsudanuma, Chiba 2750016, Japan Chiba Inst Technol 2-17-1 Tsudanuma Chiba Japan 2750016 , Japan
Citazione:
W.S. Park et al., "Inhibition of HIV-1 replication by a new type of circular dumbbell RNA/DNAchimeric oligonucleotides", BIOC BIOP R, 270(3), 2000, pp. 953-960

Abstract

We have designed a new class of oligonucleotides, "dumbbell RNA/DNA chimeric phosphodiesters," containing two alkyl loop structures with RNA/DNA basepairs [sense (RNA) and antisense (DNA)] in the double helical stem. The reaction of nicked (NDRDON-gag-AUG) and circular (CDRDON-gag-AUG) dumbbell RNA/DNA chimeric oligonucleotides with RNaseH gave the corresponding antisense phosphodiester oligonucleotide together with the sense RNA cleavage products. The liberated antisense phosphodiester oligodeoxynucleotide was bound to the target RNA. The circular dumbbell RNA/DNA chimeric oligonucleotide showed more nuclease resistance and cellular uptake than the linear antisense phosphorothioate oligodeoxynucleotide (S-ODN-gag-AUG) and nicked dumbbellRNA/DNA chimeric oligonucleotide. The CDRDON-gag-AUG with an AUG initiation codon sequence, as the target of the HIV-1 gag-gene (779-801), was synthesized and tested for inhibitory effects using peripheral blood mononuclear cells. The circular dumbbell RNA/DNA chimeric oligonucleotide (CDRDON-gag-AUG) showed highly inhibitory effects compared to the antisense phosphorothioate oligonucleotide (S-ODN-gag-AUG), indicating sequence-specific inhibition of HIV-1 replication without the inhibition of reverse transcriptase and/or the viral entry process such as antisense phosphorothioate oligonucleotides. (C) 2000 Academic Press.

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Documento generato il 28/09/20 alle ore 14:52:39