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Titolo:
Early pregnancy factor is required at two important stages of embryonic development in the mouse
Autore:
Athanasas-Platsis, S; Corcoran, CM; Kaye, PL; Cavanagh, AC; Morton, H;
Indirizzi:
Univ Queensland, Dept Surg, Royal Brisbane Hosp, Brisbane, Qld 4029, Australia Univ Queensland Brisbane Qld Australia 4029 Brisbane, Qld 4029, Australia Univ Queensland, Dept Physiol & Pharmacol, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia
Titolo Testata:
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 4, volume: 43, anno: 2000,
pagine: 223 - 233
SICI:
1046-7408(200004)43:4<223:EPFIRA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; BLASTOCYST IMPLANTATION; PREIMPLANTATION EMBRYOS; MONOCLONAL-ANTIBODIES; SUPPRESSOR FACTOR; GROWTH-FACTORS; IN-VITRO; MICE; EXPRESSION; RECEPTOR;
Keywords:
embryonic development; implantation; passive immunization; trophoblast outgrowth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Athanasas-Platsis, S Univ Queensland, Dept Surg, Royal Brisbane Hosp, ClinSci Bldg, Brisbane, Qld 4029, Australia Univ Queensland Clin Sci Bldg Brisbane Qld Australia 4029
Citazione:
S. Athanasas-Platsis et al., "Early pregnancy factor is required at two important stages of embryonic development in the mouse", AM J REPROD, 43(4), 2000, pp. 223-233

Abstract

PROBLEM: The importance of early pregnancy factor (EPF) at the pre-implantation stage of development (days 1-3 post-coitum [p.c.]) has been previously established in this laboratory. However, the role of EPF at the implantation stage (days 4.5-5 p.c.) has not been determined. This present study therefore investigates the role of EPF at this important developmental stage, both in vivo and in vitro. METHOD OF STUDY: Mated mice were passively immunized with anti-EPF antibodies at the peri-implantation stage (days 3.5-4 p.c.) and embryo implantation recorded. Parallel studies were conducted in vitro, where the effect of anti-EPF antibodies on trophoblast outgrowth of blastocysts was determined. RESULTS: Administration of anti-EPF antibodies in vivo at the peri-implantation stage of development resulted in failure of embryos to implant. Similarly, trophoblastic outgrowth of blastocysts was adversely affected in the presence of anti-EPF antibodies. CONCLUSIONS: These results, together with previous findings that anti-EPF antibodies retard embryonic development when administered at the early pre-implantation stage, clearly demonstrate that EPF is required by the embryo at two important developmental stages - the one-two-cell stage and the peri-implantation stage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:16:35