Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Simultaneous quantification of various retinoids by high performance liquid chromatography: Its relevance to alcohol research
Autore:
Yokoyama, H; Matsumoto, M; Shiraishi, H; Ishii, H;
Indirizzi:
Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, Tokyo 160, Japan Keio Univ Tokyo Japan 160 pt Internal Med, Shinjuku Ku, Tokyo 160, Japan
Titolo Testata:
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
fascicolo: 4, volume: 24, anno: 2000, supplemento:, S
pagine: 26S - 29S
SICI:
0145-6008(200004)24:4<26S:SQOVRB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID;
Keywords:
retinol; retinoic acid; photoisomerization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
6
Recensione:
Indirizzi per estratti:
Indirizzo: Yokoyama, H Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, 35 Shinanomachi, Tokyo160, Japan Keio Univ 35 Shinanomachi Tokyo Japan 160 hi, Tokyo160, Japan
Citazione:
H. Yokoyama et al., "Simultaneous quantification of various retinoids by high performance liquid chromatography: Its relevance to alcohol research", ALC CLIN EX, 24(4), 2000, pp. 26S-29S

Abstract

Background: We established a high performance liquid chromatography systemthat allowed simultaneous quantification of various retinoids. Methods: We applied the retinoids to a high performance liquid chromatography system with a silica gel absorption column. Samples were separated by the system with a binary multistep gradient with two kinds of solvent that contained n-Hexan, 2-propanol, and glacial acetic acid in different ratios. Each retinoid was detected at a wavelength of 350 nm. Results: This condition allowed separation of 13-cis-retinoic acid, 9-cis-retinoic acid, all-trans-retinoic acid, 13-cis-retinol, all-trans-retinol, all-trans-4-oxo-retinoic acid, and 13-cis-4-oxo-retinoic acid as distinct single peaks. Each retinoid was also analyzed separately and its retention time determined. To ascertain the reliability of this system for retinoid quantification, retinoids at various concentrations were applied to the system. We observed the linearities between the concentration and area under thecurve of the peak for each retinoid by linear least-squares regression analysis up to 2.5 ng/ml for all retinoic acids and up to 5 ng/ml for all retinols. There was no significant scattering in tests of within-day reproducibility or day-to-day reproducibility. Using this system, we examined effectsof light exposure on isomerization of retinoids. When retinoids were exposed to room light for 2 hr, the amounts of all but 13-cis-retinol changed significantly. In particular, the amounts of all-trans-retinoic acid and 9-cis-retinoic acid were reduced by 40% and 60%, respectively. Conclusion: The HPLC system established in this study should be useful forstudying the oxidation pathway of retinol to retinoic acid. A light-shielded condition is required when particular retinoic acids are analyzed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:57:35