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Titolo:
Lymphoid organs as a major reservoir for human T-cell leukemia virus type 1 in experimentally infected squirrel monkeys (Saimiri sciureus): Provirus expression, persistence, and humoral and cellular immune responses
Autore:
Kazanji, M; Ureta-Vidal, A; Ozden, S; Tangy, F; de Thoisy, B; Fiette, L; Talarmin, A; Gessain, A; de The, G;
Indirizzi:
Inst Pasteur de la Guyane, Lab Retrovirol, Cayenne 97300, French Guiana Inst Pasteur de la Guyane Cayenne French Guiana 97300 300, French Guiana Inst Pasteur de la Guyane, Ctr Primatol, Cayenne 97300, French Guiana InstPasteur de la Guyane Cayenne French Guiana 97300 300, French Guiana Inst Pasteur, Unite Oncol Virale, F-75724 Paris, France Inst Pasteur Paris France F-75724 te Oncol Virale, F-75724 Paris, France Inst Pasteur, Unite Virus Lents, F-75724 Paris, France Inst Pasteur Paris France F-75724 ite Virus Lents, F-75724 Paris, France Inst Pasteur, CNRS URA D1930, Unite Immunite Cellulaire Antivirale, F-75724 Paris, France Inst Pasteur Paris France F-75724 aire Antivirale, F-75724 Paris, France Inst Pasteur, Unite Histopathol, F-75724 Paris, France Inst Pasteur Paris France F-75724 ite Histopathol, F-75724 Paris, France
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 10, volume: 74, anno: 2000,
pagine: 4860 - 4867
SICI:
0022-538X(200005)74:10<4860:LOAAMR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROPICAL SPASTIC PARAPARESIS; POLYMERASE CHAIN-REACTION; HTLV-I INFECTION; BLOOD MONONUCLEAR-CELLS; ANTIBODY-RESPONSE; HIV-INFECTION; GENE; LYMPHOCYTES; DISEASE; RATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Kazanji, M Inst Pasteur de la Guyane, Lab Retrovirol, BP 6010,23 Ave Pasteur, Cayenne97300, French Guiana Inst Pasteur de la Guyane BP 6010,23 Ave Pasteur Cayenne French Guiana 97300
Citazione:
M. Kazanji et al., "Lymphoid organs as a major reservoir for human T-cell leukemia virus type 1 in experimentally infected squirrel monkeys (Saimiri sciureus): Provirus expression, persistence, and humoral and cellular immune responses", J VIROLOGY, 74(10), 2000, pp. 4860-4867

Abstract

The aim of this study was to investigate the distribution of human T-cell leukemia virus type 1 (HTLV-1) in various organs of serially sacrificed squirrel monkeys (Saimiri sciureus) in order to localize the reservoir of the virus and to evaluate the relationship between viral expression and the humoral or cellular immune response during infection. Six squirrel monkeys infected with HTLV-1 were sacrificed 6, 12, and 35 days and 3, 6, and 26 months after inoculation and 20 organs and tissues were collected from each animal, PCR and reverse transcription-PCR (RT-PCR) were performed with gag and tar primers. Proviral DNA was detected by PCR in peripheral blood mononuclear cells (PBMCs) of monkeys sacrificed 6 days after inoculation and in PBMCs, spleens, and lymph nodes of monkeys sacrificed 12 and 35 days and 3, 6, and 26 months after inoculation. Furthermore, tar/rex mRNA was detected by RT-PCR in the PBMCs of two monkeys 8 to 12 days after inoculation and in the spleens and lymph nodes of the monkey sacrificed on day 12, in this animal, scattered HTLV-1 tax/rex mRNA-positive lymphocytes were detected by in situ hybridization in frozen sections of the spleen, around the germinal centers and close to the arterial capillaries. Anti-HTLV-1 tell-mediated immunity was evaluated at various times after inoculation. Anti-p40(Tax) and anti-Env cytolytic T-cell responses were detected 2 months after infection andremained detectable thereafter. When Tax peptides were used, this responseappeared to be directed against various Tax epitopes, Our results indicatethat squirrel monkeys represent a promising animal model for studying the early events of HTLV-1 infection and for evaluating candidate vaccines against HTLV-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:26:57