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Titolo:
CD8(+)-cell antiviral factor activity is not restricted to human immunodeficiency virus (HIV)-specific T cells and can block HIV replication after initiation of reverse transcription
Autore:
Le Borgne, S; Fevrier, M; Callebaut, C; Lee, SP; Riviere, Y;
Indirizzi:
Inst Pasteur, Lab Immunopathol Virale, URA CNRS 1930, Dept Retrovirus, F-75724 Paris 15, France Inst Pasteur Paris France 15 , Dept Retrovirus, F-75724 Paris 15, France Univ Birmingham, CRC, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England Univ Birmingham Birmingham W Midlands England B15 2TA W Midlands, England
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 10, volume: 74, anno: 2000,
pagine: 4456 - 4464
SICI:
0022-538X(200005)74:10<4456:CAFAIN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG TERMINAL REPEAT; EPSTEIN-BARR-VIRUS; CYTOTOXIC LYMPHOCYTES-T; INFECTED CD4+ CELLS; CD8+ CELLS; CLINICAL STATE; SUPPRESSION; TYPE-1; INDIVIDUALS; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Riviere, Y Inst Pasteur, Lab Immunopathol Virale, URA CNRS 1930, Dept Retrovirus, 28 Rue Dr Roux, F-75724 Paris 15, France Inst Pasteur 28 Rue Dr Roux Paris France 15 4 Paris 15, France
Citazione:
S. Le Borgne et al., "CD8(+)-cell antiviral factor activity is not restricted to human immunodeficiency virus (HIV)-specific T cells and can block HIV replication after initiation of reverse transcription", J VIROLOGY, 74(10), 2000, pp. 4456-4464

Abstract

CD8(+) lymphocytes from human immunodeficiency virus (HV)-infected patients can suppress in vitro HIV replication in CD4(+) T cells by a noncytolyticmechanism involving secreted CDS'-cell antiviral factor(s) (CAF). Using anHIV Nef-specific cytotoxic-T-lymphocyte (CTL) line and autologous CD4(+) Tcells infected with a nef-deleted HIV-1 virus, we demonstrated that, aftera priming antigenic stimulation, this suppression does not require the presence of the specific antigen during the effector phase. Furthermore, usingan Epstein-Barr virus (EBV)-specific CTL line from an HIV-seronegative donor, we demonstrated that the ability to inhibit HIV replication in a noncytolytic manner is not restricted to Hn:-specific effector cells; indeed, EBV-specific CTL were as efficient as HN-specific effectors in suppressing R5 or X4 HIV-1 strain replication in vitro. This HIV-suppressive activity mediated by a soluble factor(s) present in the culture supernatant was detectable for up to 14 days following stimulation of EBV-specific CD8(+) cells with the cognate epitope peptide. Following acute infection of CEM cells with an X4 strain of HIV-1, EBV-specific CTL line supernatant containing AN-suppressive activity did not block virus entry but was shown to interfere with virus replication after the first template snitching of reverse transcription. Our results suggest that the noncytolytic control of HIV replication byEBV-specific CD8(+) T lymphocytes corresponded to a CAF-like activity and thus demonstrate that CAF. production may not be restricted to CTL induced during HIV disease. Moreover, CAF acts after reverse transcription at leastfor X4 isolate replication inhibition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:17:41