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Titolo:
MULTILOCUS DNA FINGERPRINTS IN THE PLANT CYNOGLOSSUM-OFFICINALE L ANDTHEIR USE IN THE ESTIMATION OF SELFING
Autore:
VRIELING K; SAUMITOULAPRADE P; MEELIS E; EPPLEN JT;
Indirizzi:
INST EVOLUTIONARY & ECOL SCI,VAN DER KLAAUW LAB,POB 9516 NL-2300 RA LEIDEN NETHERLANDS UNIV LILLE,LAB GENET & EVOLUT POPULAT VEGETALES,CNRS,URA 1185 F-59655VILLENEUVE DASCQ FRANCE RUHR UNIV BOCHUM D-44780 BOCHUM GERMANY
Titolo Testata:
Molecular ecology
fascicolo: 6, volume: 6, anno: 1997,
pagine: 587 - 593
SICI:
0962-1083(1997)6:6<587:MDFITP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
INBREEDING DEPRESSION; OUTCROSSING RATES; SIZE;
Keywords:
MULTILOCUS DNA FINGERPRINTING (GATA)(4); SYNTHETIC OLIGONUCLEOTIDE PROBES; CYNOGLOSSUM OFFICINALE; HOUNDS-TONGUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K. Vrieling et al., "MULTILOCUS DNA FINGERPRINTS IN THE PLANT CYNOGLOSSUM-OFFICINALE L ANDTHEIR USE IN THE ESTIMATION OF SELFING", Molecular ecology, 6(6), 1997, pp. 587-593

Abstract

We have developed a nonradioactive oligonucleotide multilocus DNA fingerprinting method for Cynoglossum officinale. Of the 19 probes tested, six probes yielded banding patterns for all restriction enzymes used. All but one of the informative probes are repeats with a four-base motif. Approximately 60% of the loci appeared to be polymorphic. The sensitivity of the nonradioactive method was equal to that of the radioactive method. In addition, a new simple calculation method is presented to estimate selfing rates and approximate 95% confidence limits fromthe DNA fingerprint profiles avoiding 'between-gel' comparisons. The selfing rates differed significantly (as determined from 95% confidence intervals) between naturally pollinated individuals of C. officinalewithin the experimental population. The estimates ranged from 0 to 70% selfing.

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Documento generato il 01/10/20 alle ore 01:30:08