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Titolo:
Characterization of superantigen-induced clonal deletion with a novel clanIII-restricted avian monoclonal antibody: Exploiting evolutionary distanceto create antibodies specific for a conserved V-H region surface
Autore:
Cary, SP; Lee, J; Wagenknecht, R; Silverman, GJ;
Indirizzi:
Univ Calif San Diego, Dept Med, Sam & Rose Stein Inst Res Aging, La Jolla,CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 s Aging, La Jolla,CA 92093 USA Univ Calif San Diego, Dept Med, Theodore Gildred Canc Ctr, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 nc Ctr, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 9, volume: 164, anno: 2000,
pagine: 4730 - 4741
SICI:
0022-1767(20000501)164:9<4730:COSCDW>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
STAPHYLOCOCCAL PROTEIN-A; B-CELL SUPERANTIGEN; HEAVY-CHAIN LOCUS; ANTIGEN-BINDING; POSSIBLE ROLES; GENE FAMILY; HUMAN-IGM; CHICKEN; IMMUNOGLOBULINS; SIALOPROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Silverman, GJ Univ Calif San Diego, Dept Med 0663, 9500 Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 SA
Citazione:
S.P. Cary et al., "Characterization of superantigen-induced clonal deletion with a novel clanIII-restricted avian monoclonal antibody: Exploiting evolutionary distanceto create antibodies specific for a conserved V-H region surface", J IMMUNOL, 164(9), 2000, pp. 4730-4741

Abstract

Evolution of the Ab system has yielded three clam of V-H region genes thatare represented in almost every known higher species with an adaptive immune system. These clans are defined by sequence homologies primarily in highly conserved framework (FR) subdomains, which serve a scaffolding function maintaining the conformation of loops responsible for Ag binding. Structural analyses indicate that the V(H)F1 and FR3 form a conserved composite exposed surface, which has been implicated in interactions with B cell superantigens. To directly investigate the expression of dan-defined supraclonal sets, we exploited the evolutionary distance of the chicken immune system andthe selection power of phage display, to derive Abs diagnostic for clan III Ig. Using a specially tailored immunization and selection strategy, we created recombinant avian single chain Fv Abs specific for the clan III products, including those from the human V(H)3 family, and the analogous murine 7183, S107, J606, X24, and DNA4 families, and binding was competitive with natural B cell superantigens, The archetype, LJ-26, was demonstrated to recognize a clan-specific surface expressed in diverse mammalian, and also theXenopus and chicken, immune systems. In flow-cytometric studies with LJ-26, we found that treatment of heterozygous T15i transgenic mice with a modelB cell superantigen induced a dan m-restricted clonal deletion. These studies demonstrate the utility of a novel recombinant serologic reagent to study the composition of the B cell compartment and also the consequences of Bcell superantigen exposure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:15:15