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Titolo:
Blockade of cadherin-6B activity perturbs the distribution of PSD-95 family proteins in retinal neurones
Autore:
Honjo, Y; Nakagawa, S; Takeichi, M;
Indirizzi:
Kyoto Univ, Grad Sch Biostudies, Dept Cell & Dev Biol, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Dev Biol, Sakyo Ku, Kyoto 6068502, Japan Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England Univ Cambridge Cambridge England CB2 3DY nat, Cambridge CB2 3DY, England
Titolo Testata:
GENES TO CELLS
fascicolo: 4, volume: 5, anno: 2000,
pagine: 309 - 318
SICI:
1356-9597(200004)5:4<309:BOCAPT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-ADHESION MOLECULES; LONG-TERM POTENTIATION; JUNCTIONAL COMPLEX; EPITHELIAL-CELLS; ALPHA-CATENIN; OPTIC TECTUM; EXPRESSION; DOMAIN; SPECIFICITIES; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Takeichi, M Kyoto Univ, Grad Sch Biostudies, Dept Cell & Dev Biol, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 akyo Ku, Kyoto 6068502, Japan
Citazione:
Y. Honjo et al., "Blockade of cadherin-6B activity perturbs the distribution of PSD-95 family proteins in retinal neurones", GENES CELLS, 5(4), 2000, pp. 309-318

Abstract

Background: Synaptic junctions have cadherin-catenin complexes, but their functions are poorly understood. Using retinal neurones, we investigated the role of this adhesion machinery in synaptic organization. Results: In cultures of chicken retinal cells, cadherin-6B (cad6B) and cadherin-7 (cad7) are expressed by distinct neurones, each being distributed in a punctate pattern along their neurites as well as in the soma. Double-immunostaining for cad6B and PSD-95/SAP90 or other PSD-95 family members, known to localize in the postsynaptic density, showed that their distributionsoverlapped each other. To assess the role for cad6B, we incubated retinal cells with antibodies that could specifically block cad6B-mediated adhesion. In the antibody-treated neurones, the localization pattern of PSD-95 family proteins was altered, that is, their staining signals tended to be reduced or disarranged. We then examined whether cadherins interacted molecularly with PSD-95: Cadherin immunoprecipitates from brain lysates did not contain PSD-95; nevertheless, this protein was co-precipitated with alpha N- andbeta-catenins. When PSD-95 proteins were ectopically expressed in epithelial cells, some of these molecules were concentrated in cell-cell junctions,co-localizing with E-cadherin, and this junctional localization of PSD-95 was abolished by blocking of E-cadherin activity. Conclusion: These results suggest that cadherins play a role in the subcellular organization of postsynaptic density components through some, perhapsindirect, molecular interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:33:09