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Titolo:
Effective ex vivo generation of granulopoietic postprogenitor cells from mobilized peripheral blood CD34(+) cells
Autore:
Scheding, S; Meister, B; Buhring, HJ; Baum, CM; McKearn, JP; Bock, T; Kanz, T; Brugger, W;
Indirizzi:
Univ Tubingen, Dept Internal Med, Div Hematol Oncol Rheumatol & Immunol, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 mmunol, D-72076 Tubingen, Germany Monsanto Co, Searle Res & Dev, St Louis, MO USA Monsanto Co St Louis MO USA santo Co, Searle Res & Dev, St Louis, MO USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 4, volume: 28, anno: 2000,
pagine: 460 - 470
SICI:
0301-472X(200004)28:4<460:EEVGOG>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DOSE CHEMOTHERAPY; HEMATOPOIETIC PROGENITOR CELLS; FLOW CYTOMETRIC ANALYSIS; HUMAN BONE-MARROW; NEUTROPHIL MATURATION; EXVIVO EXPANSION; MYELOID CELLS; BREAST-CANCER; LUNG-CANCER; TRANSPLANTATION;
Keywords:
ex vivo expansion; granulopoietic postprogenitor cells; high-dose chemotherapy; peripheral blood progenitor cells; transplantation; CD34(+); hematopoietic progenitor cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Scheding, S Univ Tubingen, Dept Internal Med, Div Hematol Oncol Rheumatol & Immunol, Otfried Muller Str 10, D-72076 Tubingen, Germany Univ Tubingen Otfried Muller Str 10 Tubingen Germany D-72076
Citazione:
S. Scheding et al., "Effective ex vivo generation of granulopoietic postprogenitor cells from mobilized peripheral blood CD34(+) cells", EXP HEMATOL, 28(4), 2000, pp. 460-470

Abstract

Objective. Neutropenia following high-dose chemotherapy and peripheral blood progenitor cell (PBPC) transplantation might be abrogated by an additional transplantation of ex vivo generated granulopoietic postprogenitor cells(GPPC), Therefore, the ex vivo expansion of CD34(+) PBPC was systematically studied aiming for optimum GPPC production,Materials and Methods. CD34(+) PBPC were cultured in serum-free medium comparing different (n = 32) combinations of stem cell factor (S), interleukin1 (1), interleukin 3 (IL-3) (3), interleukin-6 (6), erythropoietin (E), granulocyte colony-stimulating factor (G), granulate-macrophage colony-stimulating factor (GM), daniplestim (D, a novel IL-3 receptor agonist), and Flt3ligand (FL) under various culture conditions. Ex vivo generated cells wereassessed by flow cytometry, morphology, and progenitor cell assays,Results. Addition of G +/- GM but not GM alone to cultures stimulated withS163E effectively induced the generation of GPPC, GPPC production was maximum after 12 to 14 days. Best expansion rates were observed when tells werecultured at 1.5 x 10(4)/mL in 21% O-2. Modifications of culture conditionsHere either less or equally effective (i.e., modification of starting cellconcentrations, low oxygen, addition of serum albumin or autologous plasma, repetitive feeding). Comparison of different cytokine combinations revealed that the optimum GPPC expansion cocktail consisted of S6GD + FL (day 12:130-fold cellular expansion, 32% myeloblasts/promyelocytes, 49.4% myelocytes/metamyelocytes, 12.4% bands/segmented), which furthermore expanded CD34() cells (3.4-fold) and clonogenic progenitors (13.4-fold). Conclusion. Using the S6DG + FL expansion cocktail, GPPC could be effectively produced es vivo starting from positively selected CD34 PBPC, possibly enabling amelioration or even abrogation of posttransplant neutropenia, (C)2000 International Society for Experimental Hematology. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:15:50