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Titolo:
Two RAPD markers linked to a major fertility restorer gene in pepper
Autore:
Zhang, BX; Huang, SW; Yang, GM; Guo, JZ;
Indirizzi:
Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples RChina Chinese Acad Agr Sci Beijing Peoples R China 100081 0081, Peoples RChina
Titolo Testata:
EUPHYTICA
fascicolo: 2, volume: 113, anno: 2000,
pagine: 155 - 161
SICI:
0014-2336(2000)113:2<155:TRMLTA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOPLASMIC MALE-STERILITY; RESISTANCE GENES; LINKAGE MAP; IDENTIFICATION; CAPSICUM; CONSTRUCTION; TOMATO; L.;
Keywords:
bulked segregant analysis; fertility restorer gene; marker assisted selection (MAS); pepper (Capsicum annuum L.); random amplified polymorphic DNA (RAPD);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Huang, SW Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081,Peoples RChina Chinese Acad Agr Sci Beijing Peoples R China 100081 les RChina
Citazione:
B.X. Zhang et al., "Two RAPD markers linked to a major fertility restorer gene in pepper", EUPHYTICA, 113(2), 2000, pp. 155-161

Abstract

Both major and minor genes control the restoring of fertility in the cytoplasmic male-sterility system in pepper (Capsicum annuum L.). Bulked segregant analysis (BSA) was applied to identify molecular markers linked to a major restorer gene (Rf) using the F-2 population of Niujiaojiao No.21 (rfrf)/Xiangtanwan (RfRf). Two random amplified polymorphic DNA (RAPD) markers linked to this allele were detected with 520 decamer primers with arbitrary sequences. OP13(1400) is a tightly linked marker with a genetic distance of 0.37 cm. OW19(800) is on the opposite side with a distance of 8.12 cm. Both markers were repeatable and easy to score. A panel of genotypes, including 13 elite inbred lines with different fertility restoring ability, were assayed for the presence of OP13(1400) and OW19(800). The markers are absent inall sweet pepper lines, indicating that they will be most helpful for transferring Rf into sweet pepper lines. With the aid of these markers, the size of the backcross population for testcrosses can be minimized. Furthermore, these markers will be useful in genetic analysis of the minor genes.

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Documento generato il 04/12/20 alle ore 01:33:10