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Titolo:
Nek2B a novel maternal form of Nek2 kinase, is essential for the assembly or maintenance of centrosomes in early Xenopus embryos
Autore:
Uto, K; Sagata, N;
Indirizzi:
Kyushu Univ, Grad Sch Sci, Dept Biol, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 Sci, Dept Biol, Fukuoka 8128581, Japan
Titolo Testata:
EMBO JOURNAL
fascicolo: 8, volume: 19, anno: 2000,
pagine: 1816 - 1826
SICI:
0261-4189(20000417)19:8<1816:NANMFO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUBULIN RING COMPLEX; CELL-CYCLE; GAMMA-TUBULIN; PROTEIN-KINASE; ASPERGILLUS-NIDULANS; EGG EXTRACTS; IN-VITRO; MICROTUBULE ORGANIZATION; DROSOPHILA-MELANOGASTER; MITOTIC REGULATOR;
Keywords:
cell cycle; centrosome; Nek2; spindle formation; Xenopus embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Sagata, N Kyushu Univ, Grad Sch Sci, Dept Biol, Hakozaki 6-10-1, Fukuoka 8128581, Japan Kyushu Univ Hakozaki 6-10-1 Fukuoka Japan 8128581 128581, Japan
Citazione:
K. Uto e N. Sagata, "Nek2B a novel maternal form of Nek2 kinase, is essential for the assembly or maintenance of centrosomes in early Xenopus embryos", EMBO J, 19(8), 2000, pp. 1816-1826

Abstract

Nek2, a NIMA-related kinase, has been postulated to play a role in both the meiotic and mitotic cell cycles in vertebrates. Xenopus has two Nek2 splice variants, Nek2A and Nek2B, which are zygotic and maternal forms, respectively. Here we have examined the role of Nek2B in oocyte meiosis and early embryonic mitosis. Specific inhibition of Nek2B function does not interferewith the oscillation of Cdc2 activity in either the meiotic or mitotic cell cycles; however, it does cause abortive cleavage of early embryos, in which bipolar spindle formation is severely impaired due to fragmentation or dispersal of the centrosomes, to which endogenous Nek2B protein localizes. In contrast, inhibition of Nek2B function does not affect meiotic spindle formation in oocytes, in which functional centrosomes are absent. Thus, strikingly, Nek2B is specifically required for centrosome assembly and/or maintenance (and hence for normal bipolar spindle formation and cleavage) in early Xenopus embryos. Finally, (ectopic) Nek2A but not NeK2B is very labile incleaving embryos, suggesting that Nek2A cannot replace the centrosomal function of Nek2B in early embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:16:58