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Titolo:
Newly developed encapsulation-dehydration protocol for plant cryopreservation
Autore:
Sakai, A; Matsumoto, T; Hirai, D; Niino, T;
Indirizzi:
Hokkaido Univ, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 iv, Sapporo, Hokkaido 060, Japan Shimane Agr Expt Stn, Izumo, Shimane 6930035, Japan Shimane Agr Expt Stn Izumo Shimane Japan 6930035 , Shimane 6930035, Japan Hokkaido Prefectural Plant Genet Resources Ctr, Takinokawa, Takikawa 0730013, Japan Hokkaido Prefectural Plant Genet Resources Ctr Takinokawa Takikawa Japan 0730013 Tohoku Natl Agr Expt Stn, Dept Upland Farming, Fukushima 9602156, Japan Tohoku Natl Agr Expt Stn Fukushima Japan 9602156 ukushima 9602156, Japan
Titolo Testata:
CRYO-LETTERS
fascicolo: 1, volume: 21, anno: 2000,
pagine: 53 - 62
SICI:
0143-2044(200001/02)21:1<53:NDEPFP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWN SHOOT TIPS; WASABI WASABIA-JAPONICA; VAR BRASILIENSIS TANAKA; DAUCUS-CAROTA L; APICAL MERISTEMS; VITRIFICATION METHOD; SOMATIC EMBRYOS; NUCELLAR CELLS; SURVIVAL; INVITRO;
Keywords:
cryopreservation; new encapsulation-dehydration protocol; vitrification protocol; chrysanthemum; mint; wasabi;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Sakai, A Asabucho 1-5-23,Kita Ku, Sapporo, Hokkaido 0010045, Japan Asabucho 1-5-23,Kita Ku Sapporo Hokkaido Japan 0010045 45, Japan
Citazione:
A. Sakai et al., "Newly developed encapsulation-dehydration protocol for plant cryopreservation", CRYO-LETT, 21(1), 2000, pp. 53-62

Abstract

A simplified and efficient encapsulation-dehydration protocol, which is a compromise between vitrification and encapsulation - dehydration, was presented for plant cryopreservation. In this protocol, during the encapsulationprocess, the apices precultured with 0.3 M sucrose for 16 h were simultaneously osmoprotected with a mixture of square 2 M glycerol plus 0.4 M sucrose for 1 h. These encapsulated apices were directly dehydrated with dry silica gel prior to a plunge into LN without the pretreatment of 0.8M sucrose for 16 h. This protocol produced much higher rates of recovery growth in thethree plant species tested (wasabi, chrysanthemum, and mint) than those cryopreserved by the conventional encapsulation-dehydration. This protocol also considerably reduced the time needed for the cryogenic procedure. Thus, this new protocol appears promising for cryopreservation of shoot apices and other explants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:15:13