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Titolo:
Opioid and cannabinoid receptors share a common pool of GTP-binding proteins in cotransfected cells, but not in cells which endogenously coexpress the receptors
Autore:
Shapira, M; Vogel, Z; Sarne, Y;
Indirizzi:
Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 rmacol, IL-69978 Tel Aviv, Israel Weizmann Inst Sci, Dept Neurobiol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 biol, IL-76100 Rehovot, Israel Tel Aviv Univ, Sackler Fac Med, Mauerberger Chair Neuropharmacol, IL-69978Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 armacol, IL-69978Tel Aviv, Israel
Titolo Testata:
CELLULAR AND MOLECULAR NEUROBIOLOGY
fascicolo: 3, volume: 20, anno: 2000,
pagine: 291 - 304
SICI:
0272-4340(200006)20:3<291:OACRSA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROBLASTOMA SH-SY5Y CELLS; II ADENYLYL-CYCLASE; MULTIPLE G-PROTEINS; FUNCTIONAL EXPRESSION; CALCIUM CHANNELS; PHOSPHOLIPASE-C; HYBRID-CELLS; MU; ACTIVATION; MEMBRANES;
Keywords:
opioid receptors; cannabinoid receptors; GTP-binding proteins; cell transfection; [S-35]GTP gamma S binding; neuroblastoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Sarne, Y Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 L-69978 Tel Aviv, Israel
Citazione:
M. Shapira et al., "Opioid and cannabinoid receptors share a common pool of GTP-binding proteins in cotransfected cells, but not in cells which endogenously coexpress the receptors", CELL MOL N, 20(3), 2000, pp. 291-304

Abstract

Opioid (mu. delta, kappa) and cannabinoid (CB1, CB2) receptors are coupledmainly to G(i)/G(o) GTP-binding proteins. The goal of the present study was to determine whether different subtypes of opioid and cannabinoid receptors, when coexpressed in the same cell, share a common reservoir. or utilizedifferent pools, of G proteins. The stimulation of [S-35]GTP gamma S binding by selective opioid and cannabinoid agonists was tested in transiently transfected COS-7 cells, as well as in neuroblastoma cell lines. In COS-7 cells, cotransfection of mu- and delta-opioid receptors led to stimulation of [S-35]GTP gamma S binding by either mu-selective (DAMGO) or delta-selective (DPDPE) agonists. The combinedeffect of the two agonists was similar to the effect of either DAMGO or DPDPE alone, suggesting the activation of a common G-protein reservoir by thetwo receptor subtypes. The same phenomenon was observed when COS-7 cells were cotransfected with CB1 cannabinoid receptors and either mu- or delta-opioid receptors. On the other hand. in N18STG2 neuroblastoma cells, which endogenously coexpress CB1 and delta-opioid receptors, as well as in SK-N-SH neuroblastoma cells, which coexpress mu- and delta-opioid receptors, the combined effects of the various agonists (the selective cannabinoid DALN and the selective opioids DPDPE and DAMGO) were additive, implying the activation of differentpools of G proteins by each receptor subtype. These results suggest a fundamental difference between native and artificially transfected cells regarding the compartmentalization of receptors and GTP-binding proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:10:24