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Titolo:
Characterization of the Aspergillus parasiticus major nitrogen regulatory gene, areA
Autore:
Chang, PK; Yu, JJ; Bhatnagar, D; Cleveland, TE;
Indirizzi:
ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA ARS New Orleans LA USA 70124 Reg Res Ctr, USDA, New Orleans, LA 70124 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 1-3, volume: 1491, anno: 2000,
pagine: 263 - 266
SICI:
0167-4781(20000425)1491:1-3<263:COTAPM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFLATOXIN BIOSYNTHESIS; METABOLITE REPRESSION; TRANSCRIPTION; SPECIFICITY; NIDULANS; PROTEIN; FUNGI; AFLR;
Keywords:
nitrogen regulation; areA; DNA-binding domain; transcription activation domain; aflatoxin; Aspergillus parasiticus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, PK ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA ARS 1100 Robert E Lee Blvd New Orleans LA USA 70124 LA 70124 USA
Citazione:
P.K. Chang et al., "Characterization of the Aspergillus parasiticus major nitrogen regulatory gene, areA", BBA-GENE ST, 1491(1-3), 2000, pp. 263-266

Abstract

The major nitrogen regulatory gene, areA, was cloned from Aspergillus parasiticus. It encoded a polypeptide of 864 amino acids which contained a nuclear localization signal (NLS), a highly acidic region from positions 497 to542, a Cys-X-2-Cys-X-17-Cys-X-2-Cys DNA-binding motif and a conserved carboxy-terminus. Electrophoretic mobility shift assays suggested that the A. parasiticus AREA DNA-binding domain fusion protein bound cooperatively to single GATA elements in the A. parasiticus niaD-niiA intergenic region. AREA also bound to the aflR-qflJ intergenic region of the aflatoxin biosynthesisgene cluster. Regions of areA were fused to a yeast GAL4 DNA-binding domain coding region to localize putative transcription activation domain(s) of AREA based on activation of the GAL1(p)::lacZ reporter gene expression. Theportion between NLS and the acidic domain demonstrated 16-20-fold higher activation activities than other portions of AREA, which suggests that the transcription activation domain is located in this region. (C) 2000 ElsevierScience B.V. All rights reserved.

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Documento generato il 23/09/18 alle ore 09:22:18