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Titolo:
Genomic organization and regulation of the vav proto-oncogene
Autore:
Denkinger, DJ; Borges, CR; Butler, CL; Cushman, AM; Kawahara, RS;
Indirizzi:
Univ Nebraska, Med Ctr, Dept Pharmacol, Nebraska Med Ctr 986260, Omaha, NE68198 USA Univ Nebraska Omaha NE USA 68198 raska Med Ctr 986260, Omaha, NE68198 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 1-3, volume: 1491, anno: 2000,
pagine: 253 - 262
SICI:
0167-4781(20000425)1491:1-3<253:GOAROT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FETAL LIVER HEMATOPOIESIS; GTP EXCHANGE FACTOR; DEFINITIVE HEMATOPOIESIS; T-CELL; PROTOONCOGENE; GENE; REGION; FAMILY; EXPRESSION; THYMOCYTES;
Keywords:
vav; hematopoietic; stem cells; transcription; regulation; EGFP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Kawahara, RS Univ Nebraska, Med Ctr, Dept Pharmacol, Nebraska Med Ctr 986260, Omaha, NE68198 USA Univ Nebraska Omaha NE USA 68198 986260, Omaha, NE68198 USA
Citazione:
D.J. Denkinger et al., "Genomic organization and regulation of the vav proto-oncogene", BBA-GENE ST, 1491(1-3), 2000, pp. 253-262

Abstract

Vav and vav2 are members of the dbl family of guanine nucleotide exchange factors (GEF) for the rho/rac family of GTP binding proteins. Vav is expressed primarily in hematopoietic cells, while vav2, has a wider tissue distribution. The genomic structure of the human vav proto-oncogene was studied by identifying and sequencing all 27 exons of the gene from overlapping PI and cosmid clones. The gene spans a 77-kb region on chromosome 19. In contrast, the coding region of vav2 is distributed over 30 exons spanning 227-kb. The overall organization of the exons which encode both proteins was foundto be similar. In humans, alternative splicing of exons 6, 16 and 28 generated at least two distinct vav2 mRNA species. Several differences from the original vav cDNA sequence were noted. The most important difference was the identification of amino acid 718 as isoleucine, rather than threonine. This change warrants the reclassification of the vav SH2 domain as a type 3 SH2, instead of a type 2 SH2 as originally proposed by Songyang et al. (Mol. Cell. Biol. 14 (1994) 2777-2785). A series of vav promoter deletions were constructed using the enhanced green fluorescent protein (EGFP) as a reporter gene. A 23-bp segment that included a potential CBF/AML-1 binding site was found to be essential for ECFP expression in U937 cells. The same constructs were not active in HeLa cells, which do not express vav. A potential c-myb DNA binding site within the vav promoter was not required for EGFP expression. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 27/11/20 alle ore 03:33:37