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Titolo:
Oxidative phosphorylation supported by an alternative respiratory pathway in mitochondria from Euglena
Autore:
Moreno-Sanchez, R; Covian, R; Jasso-Chavez, R; Rodriguez-Enriquez, S; Pacheco-Moises, F; Torres-Marquez, ME;
Indirizzi:
Inst Nacl Cardiol, Dept Bioquim, Col Secc 16, Mexico City 14080, DF, Mexico Inst Nacl Cardiol Mexico City DF Mexico 14080 xico City 14080, DF, Mexico Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City 04510, DF, Mexico Univ Nacl Autonoma Mexico Mexico City DF Mexico 04510 y 04510, DF, Mexico
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
fascicolo: 3, volume: 1457, anno: 2000,
pagine: 200 - 210
SICI:
0005-2728(20000421)1457:3<200:OPSBAA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANAEROBICALLY FUNCTIONING EUKARYOTES; CYANIDE-RESISTANT RESPIRATION; ELECTRON-TRANSPORT CHAIN; GRACILIS MITOCHONDRIA; ESCHERICHIA-COLI; PLANT-MITOCHONDRIA; TERMINAL OXIDASE; ATP-SYNTHESIS; CYTOCHROME-B; ANTIMYCIN-A;
Keywords:
ATP synthesis; antimycin; cyanide-resistant respiration; Euglena;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Moreno-Sanchez, R Inst Nacl Cardiol, Dept Bioquim, Col Secc 16, Juan Badiano 1, Mexico City 14080, DF, Mexico Inst Nacl Cardiol Juan Badiano 1 MexicoCity DF Mexico 14080
Citazione:
R. Moreno-Sanchez et al., "Oxidative phosphorylation supported by an alternative respiratory pathway in mitochondria from Euglena", BBA-BIOENER, 1457(3), 2000, pp. 200-210

Abstract

The effect of antimycin, myxothiazol, 2-heytyl-4-hydroxyquinoline-N-oxide,stigmatellin and cyanide on respiration, ATP synthesis, cytochrome c reductase, and membrane potential in mitochondria isolated from dark-grown Euglena cells was determined. With L-lactate as substrate, ATP synthesis was partially inhibited by antimycin, but the other four inhibitors completely abolished the process. Cyanide also inhibited the antimycin-resistant ATP synthesis. Membrane potential was collapsed (< 60 mV) by cyanide and stigmatellin. However, in the presence of antimycin, a H+ gradient (> 60 mV) that sufficed to drive ATP synthesis remained. Cytochrome c reductase, with L-lactate as donor, was diminished by antimycin and myxothiazol. Cytochrome bc(1) complex activity was fully inhibited by antimycin. but it was resistant to myxothiazol. Stigmatellin inhibited both L-lactate-dependent cytochrome c reductase and cytochrome bc(1) complex activities. Respiration was partiallyinhibited by the five inhibitors. The cyanide-resistant respiration was strongly inhibited by diphenylamine, n-propyl-gallate, salicylhydroxamic acidand disulfiram. Based on these results, a model of the respiratory chain of Euglena mitochondria is proposed, in which a quinol-cytochrome c oxidoreductase resistant to antimycin, and a quinol oxidase resistant to antimycin and cyanide are included. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:54:07