Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Active chitinases in the apoplastic fluids of healthy white lupin (Lupinusalbus L.) plants
Autore:
Burzynski, A; Pislewska, M; Wojtaszek, P;
Indirizzi:
Polish Acad Sci, Inst Bioorgan Chem, PL-61704 Poznan, Poland Polish Acad Sci Poznan Poland PL-61704 gan Chem, PL-61704 Poznan, Poland
Titolo Testata:
ACTA PHYSIOLOGIAE PLANTARUM
fascicolo: 1, volume: 22, anno: 2000,
pagine: 31 - 38
SICI:
0137-5881(2000)22:1<31:ACITAF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; SUSPENSION-CULTURES; TOBACCO CELLS; SECRETION; BETA-1,3-GLUCANASE; BINDING; ASSAY;
Keywords:
apoplast; cell walls; chitinase; electrophoresis; intercellular washing fluid; isoelectrofocusing; Lupinus albus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Wojtaszek, P Polish Acad Sci, Inst Bioorgan Chem, Noskowskiego 12-14, PL-61704 Poznan, Poland Polish Acad Sci Noskowskiego 12-14 Poznan Poland PL-61704 nd
Citazione:
A. Burzynski et al., "Active chitinases in the apoplastic fluids of healthy white lupin (Lupinusalbus L.) plants", ACT PHYS PL, 22(1), 2000, pp. 31-38

Abstract

Acidic exocellular class III chitinase (EC 3.2.1.14) was previously identified in healthy white lupin (Lupinus albus L.) plants and suspension-cultured cells by N-terminal microsequencing. In this study, the detection of chitinase activity with Remazol Brilliant Violet 5R (RBV)-labelled chitin derivatives is described. Chitinase activity was observed in protein fractions of cytoplasmic or exocellular origin from roots, hypocotyls, cotyledons, and leaves of healthy white lupin plants. Using isoelectrofocusing followed by a new overlay technique with carboxymethyl chitin-RBV conjugate-containing gel, up to six different chitinase isoforms were visualised. Their activity was distributed fairly evenly within a plant with acidic isoforms predominating in cell walls and basic (or neutral) ones found intracellularly. Exocellular location of some chitinase isoforms were also confirmed by detection of their activities in intercellular washing fluids from white lupin tissues. Chitinase activity was demonstrated in culture filtrates and cell walls of suspension-cultured white lupin cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:05:40