Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PCR-SINGLE-STRANDED CONFIRMATIONAL POLYMORPHISM ANALYSIS FOR NON-CULTURE-BASED SUBTYPING OF MENINGOCOCCAL STRAINS IN CLINICAL SPECIMENS
Autore:
NEWCOMBE J; DYER S; BLACKWELL L; CARTWRIGHT K; PALMER WH; MCFADDEN J;
Indirizzi:
UNIV SURREY,SCH BIOL SCI,MOL MICROBIOL GRP GUILDFORD GU2 5XH SURREY ENGLAND UNIV SURREY,SCH BIOL SCI,MOL MICROBIOL GRP GUILDFORD GU2 5XH SURREY ENGLAND GLOUCESTERSHIRE ROYAL HOSP,PUBL HLTH LAB GLOUCESTER GL1 3NN ENGLAND
Titolo Testata:
Journal of clinical microbiology
fascicolo: 7, volume: 35, anno: 1997,
pagine: 1809 - 1812
SICI:
0095-1137(1997)35:7<1809:PCPAFN>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; OUTER-MEMBRANE PROTEIN; RIBOSOMAL-RNA GENE; NEISSERIA-MENINGITIDIS; CEREBROSPINAL-FLUID; DISEASE; CLASS-1; VACCINE; DNA; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
J. Newcombe et al., "PCR-SINGLE-STRANDED CONFIRMATIONAL POLYMORPHISM ANALYSIS FOR NON-CULTURE-BASED SUBTYPING OF MENINGOCOCCAL STRAINS IN CLINICAL SPECIMENS", Journal of clinical microbiology, 35(7), 1997, pp. 1809-1812

Abstract

Subspecific typing of clinical meningococcal strains is important in the investigation of outbreaks and for disease surveillance. Serogrouping, typing, and subtyping of strains currently require isolation of ameningococcus from one or more clinical specimens. However, the increasing widespread practice of preadmission administration of parenteralantibiotics has resulted in a decrease in the frequency of positive cultures obtained from blood and cerebrospinal fluid, Confirmation of meningococcal disease can be obtained by meningococcus-specific PCR from both cerebrospinal fluid (H. Ni et al., Lancet 340:1332-1434, 1992) and peripheral blood (J. Newcombe et al., J. Clin. Microbiol. 34:1637-1640, 1996) specimens, However, current PCR protocols do not yield epidemiologically useful typing information, We report here the use of PCR-single-stranded confirmational polymorphism (PCR-SSCP) analysis to amplify and type meningococcal DNA present in clinical specimens. PCR-SSCP analysis,vith the VR1 region of the Neisserin meningitidis porA gene as the target produced unique banding patterns for each serosubtype. Direct PCR-SSCP of clinical specimens can therefore provide typing data that can be used to investigate the epidemiology of clusters of cases and outbreaks and for disease surveillance in situations in which culture of patient specimens proves negative.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 12:45:28