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Titolo:
Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription
Autore:
Llopis, J; Westin, S; Ricote, M; Wang, JH; Cho, CY; Kurokawa, R; Mullen, TM; Rose, DW; Rosenfeld, MG; Tsien, RY; Glass, CK;
Indirizzi:
Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 pt Med, La Jolla, CA 92093 USA Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 rmacol, La Jolla, CA 92093 USA Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA UnivCalif San Diego La Jolla CA USA 92093 ol Med, La Jolla, CA 92093 USA Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 d Inst, La Jolla, CA 92093 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 8, volume: 97, anno: 2000,
pagine: 4363 - 4368
SICI:
0027-8424(20000411)97:8<4363:LIOCSR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEINS; COMPLEX; CBP; TRANSACTIVATION; SPECIFICITY; SUPERFAMILY; MOTIFS; GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Tsien, RY Univ Calif San Diego, Dept Med, 9500 Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 93 USA
Citazione:
J. Llopis et al., "Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription", P NAS US, 97(8), 2000, pp. 4363-4368

Abstract

Members of the nuclear receptor superfamily are thought to activate transcription by recruitment of one or more recently identified coactivator complexes, Here we demonstrate that both peroxisome proliferator-activated receptor binding protein (PBP) and steroid receptor coactivator-l (SRC-1) are required for ligand-dependent transcription of transiently transfected and chromosomally integrated reporter genes by the estrogen receptor (ER) and retinoic acid receptor (RAR), To examine ligand-dependent interactions betweennuclear receptors and specific coactivators in living cells, these proteins were tagged with cyan (CFP) and yellow (YFP) mutants of the green fluorescent protein. Fluorescence resonance energy transfer (FRET) from the CFP tothe YFP indicated interaction between the receptor and coactivator, CFP fusions to RAR or its ligand-binding domain exhibited rapid ligand-dependent FRET to YFP-tagged nuclear receptor interaction domains of the coactivatorsSRC-1 and PBP, The ER-ligand-binding domain, unlike RAR, also exhibited some basal interaction with coactivators in unstimulated cells that was abolished by the receptor antagonists tamoxifen or lCl182,780. Inhibition of FRET by tamoxifen but not lCl182,780 could be reversed by estradiol, whereas estradiol-enhanced FRET could not be inhibited by either antagonist, indicating that ligand effects can show varying degrees of hysteresis, These findings suggest that ligand-dependent transcriptional activities of the RAR andER require concurrent or sequential recruitment of SRC-1 and PBP-containing coactivator complexes.

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Documento generato il 05/12/20 alle ore 14:18:58