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Titolo:
IN-VIVO ASSESSMENT OF METABOLIC PERTURBATIONS FOLLOWING ALANINE AND GLUCAGON ADMINISTRATION USING P-31-MRS IN THE RAT
Autore:
CHANGANI KK; BARNARD ML; BELL JD; THOMAS EL; WILLIAMS SCR; BLOOM SR; ILES RA;
Indirizzi:
UNIV LONDON LONDON HOSP,COLL MED,MED UNIT,CELLULAR MECHANISMS RES GRP,TURNER ST LONDON E1 2AD ENGLAND HAMMERSMITH HOSP,ROBERT STEINER NMR UNIT LONDON W12 0HS ENGLAND HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT ENDOCRINOL LONDON W12 0HS ENGLAND UNIV LONDON QUEEN MARY & WESTFIELD COLL,DEPT CHEM,NMR UNIT LONDON E1 4NS ENGLAND
Titolo Testata:
Biochimica et biophysica acta (G). General subjects
fascicolo: 3, volume: 1335, anno: 1997,
pagine: 290 - 304
SICI:
0304-4165(1997)1335:3<290:IAOMPF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LIVER; GLUCONEOGENESIS; GLUCOSE; TRANSPORT; CYCLE;
Keywords:
ALANINE; P-31-NMR; LIVER; GLUCONEOGENESIS; IN VIVO; IN VITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
K.K. Changani et al., "IN-VIVO ASSESSMENT OF METABOLIC PERTURBATIONS FOLLOWING ALANINE AND GLUCAGON ADMINISTRATION USING P-31-MRS IN THE RAT", Biochimica et biophysica acta (G). General subjects, 1335(3), 1997, pp. 290-304

Abstract

This study set out to validate the use of P-31-NMR spectroscopy together with alanine +/- glucagon infusions to assess hepatic gluconeogenic flux in vivo. Bolus infusions of alanine (2.8 or 5.6 mmol/kg) +/- glucagon (250 mu g/kg) were used. Maximal changes in the phosphomonoesters (PME), inorganic phosphate (P-i) and beta-NTP occurred 40 mins postinfusion. PME increased 13.1% (p < 0.02) and 20.8% (P < 0.01) at 2.8 mmol/kg + glucagon and 5.6 mmol/kg +/- glucagon, respectively. P-i wasunaltered at 2.8 mmol/kg but increased by 28.8% (P < 0.01) at 5.6 mmol/kg alanine + glucagon, beta-NTP decreased by 14.4% (P < 0.02) and 16.1% (P < 0.02) at 5.6 mmol/kg -/+ glucagon, respectively. This latter infusion showed slower recovery rates of NTP which remained 12.3% (P <0.05) lower 70 min post infusion compared with pre-infusion values, P-31-NMR analysis of liver extracts revealed that PME increases were partly due to 3-phosphoglycerate and corroborated reductions in beta-NTPand gamma-NTP: beta-NDP ratio upon infusion of 5.6 mmol/kg alanine +/- glucagon. Hepatic glucose output from perfused liver experiments showed no difference between alanine concentrations indicating maximal glucose output at the lower concentration. This study has shown that in vivo P-31-NMR in combination with alanine infusion, can be used to determine metabolic changes associated with gluconeogenesis.

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Documento generato il 01/04/20 alle ore 16:44:44