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Titolo:
Scorpion neurotoxins: structure/function relationships and application in agriculture
Autore:
Froy, O; Zilberberg, N; Chejanovsky, N; Anglister, J; Loret, E; Shaanan, B; Gordon, D; Gurevitz, M;
Indirizzi:
Tel Aviv Univ, George S Wise Fac Life Sci, Dept Plant Sci, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 nt Sci, IL-69978 Tel Aviv, Israel Agr Res Org, Volcani Ctr, Inst Plant Protect, Dept Entomol, IL-50250 Bet Dagan, Israel Agr Res Org Bet Dagan Israel IL-50250 ntomol, IL-50250 Bet Dagan, Israel Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 ruct, IL-76100 Rehovot, Israel CNRS, UPR 9027, LIDSM, IBSM, F-13402 Marseille 20, France CNRS MarseilleFrance 20 9027, LIDSM, IBSM, F-13402 Marseille 20, France Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91904 -91904 Jerusalem, Israel Ctr Etud Saclay, Dept Ingenierie & Etud Prot, F-91191 Gif Sur Yvette, France Ctr Etud Saclay Gif Sur Yvette France F-91191 191 Gif Sur Yvette, France
Titolo Testata:
PEST MANAGEMENT SCIENCE
fascicolo: 5, volume: 56, anno: 2000,
pagine: 472 - 474
SICI:
1526-498X(200005)56:5<472:SNSRAA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANDROCTONUS-AUSTRALIS-HECTOR; SODIUM CURRENT INACTIVATION; FUNCTIONAL EXPRESSION; TOXIN-II; CHANNELS; PURIFICATION;
Keywords:
scorpion neurotoxin; structure-activity; baculoviruses; bioactive surface;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Gurevitz, M Tel Aviv Univ, George S Wise Fac Life Sci, Dept Plant Sci, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 9978 Tel Aviv, Israel
Citazione:
O. Froy et al., "Scorpion neurotoxins: structure/function relationships and application in agriculture", PEST MAN SC, 56(5), 2000, pp. 472-474

Abstract

Continued use of non-specific chemical insecticides poses potential risks to the environment and to human health resulting from non-target toxicity and increased insect resistance to these agents, Scorpions produce anti-insect selective polypeptide toxins that bind to and modulate voltage-sensitiveion channels in excitable tissues, thus offering alternative, environmentally safer means for insect pest control. Despite this potential, little is known about their structural elements dictating anti-insect preference, which may be useful for the design of selective insecticides. We used a bacterial system for expression and genetic dissection of two pharmacologically distinct scorpion toxins: alpha and excitatory. By exploiting a multi-disciplinary. approach consisting of mutagenesis, protein chemistry, electrophysiology, binding and toxicity assays, and structural studies, we elucidated the bioactive surface of two anti-insect toxins, Lqh alpha IT and Bj-xtrIT, In both polypeptides the bioactive surface is composed of residues surrounding the C-terminal region. In addition, a direct, immediate approach in using the toxin genes was demonstrated by engineering baculoviruses with cDNAsencoding LqhIT2 (depressant toxin), and LqhIT1 (excitatory toxin) resulting in viral vectors with significantly improved insecticidal efficacy. (C) 2000 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:43:39