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Titolo:
Responses in primary astrocytes and C6-glioma cells to ammonium chloride and dibutyryl cyclic-AMP
Autore:
Haghighat, N; McCandless, DW; Geraminegad, P;
Indirizzi:
Finch Univ Hlth Sci Chicago Med Sch, Dept Cell Biol & Anat, Chicago, IL 60064 USA Finch Univ Hlth Sci Chicago Med Sch Chicago IL USA 60064 go, IL 60064 USA
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 2, volume: 25, anno: 2000,
pagine: 277 - 284
SICI:
0364-3190(200002)25:2<277:RIPAAC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBRILLARY ACIDIC PROTEIN; PERFUSED-RAT-LIVER; HEPATOCYTE HETEROGENEITY; AMINO-ACIDS; IN-VITRO; METABOLISM; BRAIN; GLUTAMATE; TRANSPORT; SLICES;
Keywords:
GFAP; mitochondrial electron transport chain; ATP; ammonium chloride; hepatic encephalopathy; MTT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Haghighat, N Finch Univ Hlth Sci Chicago Med Sch, Dept Cell Biol & Anat, 3333 Green BayRd, Chicago, IL 60064 USA Finch Univ Hlth Sci Chicago Med Sch 3333 Green Bay Rd Chicago IL USA 60064
Citazione:
N. Haghighat et al., "Responses in primary astrocytes and C6-glioma cells to ammonium chloride and dibutyryl cyclic-AMP", NEUROCHEM R, 25(2), 2000, pp. 277-284

Abstract

Elevated brain ammonia levels are a major factor in the genesis of hepaticencephalopathy (HE). The mechanism of ammonium chloride (NH4Cl) neurotoxicity involves interruption of oxidative metabolism. This leads to decreased levels of ATP concentration and subsequent glial fibrillary acidic protein (GFAP) degradation of astrocytes and fibrous C6-glioma cells. Our study investigates NH4Cl toxicity by evaluating changes in ATP concentration and mitochondrial function as well as by evaluating alterations in GFAP expression. NH4Cl induced decreases in ATP were detected after 15 minutes in C6-glioma cells and 24 hours in both cell types. Mitochondrial function, assessed by MTT (2-4,5-dimethylthiazol A-yl)-2, 5-diphenyltetrazolium bromide) assay,was impaired in both cell types at 24 hours following NH4Cl treatment. GFAP was also significantly decreased in both cell types. Morphologic and metabolic toxicities were greater in C6-glioma cells. The data clearly indicatethat NH4Cl interrupts oxidative metabolism. The greater toxicity seen in C6-glioma cells may be due to their greater dependence on oxidative metabolism. Lastly, the decrease in GFAP is probably a consequence of diminished ATP.

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Documento generato il 25/01/20 alle ore 09:45:33