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Titolo:
Isolation of TC/AG repeat microsatellite sequences for fingerprinting riceblast fungus and their possible horizontal transfer to plant species
Autore:
Kim, NS; Park, NI; Kim, SH; Kim, ST; Han, SS; Kang, KY;
Indirizzi:
Kangweon Natl Univ, Dept Agron, Chunchon 200701, South Korea Kangweon NatlUniv Chunchon South Korea 200701 nchon 200701, South Korea Gyeongsang Natl Univ, Dept Agr Chem, Chinju 660701, South Korea GyeongsangNatl Univ Chinju South Korea 660701 hinju 660701, South Korea RDA, Inst Agr Sci & Technol, Suwon 441707, South Korea RDA Suwon South Korea 441707 gr Sci & Technol, Suwon 441707, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 2, volume: 10, anno: 2000,
pagine: 127 - 134
SICI:
1016-8478(20000430)10:2<127:IOTRMS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERSPERSED NUCLEAR-ELEMENT; MAGNAPORTHE-GRISEA; DNA-SEQUENCES; PYRICULARIA-GRISEA; POPULATION; AMPLIFICATION; PATHOGEN; ORYZAE; GENOME; LENGTH;
Keywords:
Alu-inter SSR; blast fungus; fingerprinting; horizontal gene transfer; microsatellite;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, NS Kangweon Natl Univ, Dept Agron, Chunchon 200701, South Korea Kangweon Natl Univ Chunchon South Korea 200701 0701, South Korea
Citazione:
N.S. Kim et al., "Isolation of TC/AG repeat microsatellite sequences for fingerprinting riceblast fungus and their possible horizontal transfer to plant species", MOL CELLS, 10(2), 2000, pp. 127-134

Abstract

Genome fingerprinting has been a major role in characterization of population structure and analysis of the variability in phytopathogenic fungi, In order to characterize Korean rice blast fungal isolates, the genomic DNAs were digested with AluI endonuclease and subsequent PCR amplifications usingrandom decamer primers with combinations of microsatellite primers had been carried out. This Alu-Inter SSR technique revealed high polymorphism among the Korean blast fungal isolates, Then, fragments from the Alu-Inter SSR analysis were isolated to be used as probes in Southern hybridization, which also revealed high polymorphism between isolates to distinguish individuals. The sequences of the isolated fragments contained TC/AG tandem repeats interspersed with a 30 bp direct repeat. In gel blot analysis, the isolatedTC/AG repeat microsatellite sequences were proved to be useful for characterizing the isolates in blast fungi in addition to the conventional MGR (Magnaporthe grisea repeat) probes. One interesting point was that the rice blast fungus derived TC/AG repeat microsatellite sequences were abundant in non-rice blast fungi and plant species, but not in other fungi and yeasts, Adiscussion on the possible horizontal gene transfer between phytopathogenic fungi and host plants is presented.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:53:26