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Titolo:
Pleiotropic pigmentation mutants of Porphyromonas gingivalis
Autore:
Chen, T; Dong, H; Yong, R; Duncan, MJ;
Indirizzi:
Forsyth Inst, Dept Mol Genet, Boston, MA 02115 USA Forsyth Inst Boston MAUSA 02115 st, Dept Mol Genet, Boston, MA 02115 USA
Titolo Testata:
MICROBIAL PATHOGENESIS
fascicolo: 4, volume: 28, anno: 2000,
pagine: 235 - 247
SICI:
0882-4010(200004)28:4<235:PPMOPG>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEINASE LYS-GINGIPAIN; CYSTEINE PROTEINASE; ARG-GINGIPAIN; EPITHELIAL-CELLS; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCE; SHIGELLA-FLEXNERI; MATRIX PROTEINS; ORAL-SURFACES; O-ANTIGEN;
Keywords:
Porphyromonas gingivalis; pigmentation mutants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Duncan, MJ Forsyth Inst, Dept Mol Genet, Boston, MA 02115 USA Forsyth Inst Boston MA USA 02115 l Genet, Boston, MA 02115 USA
Citazione:
T. Chen et al., "Pleiotropic pigmentation mutants of Porphyromonas gingivalis", MICROB PATH, 28(4), 2000, pp. 235-247

Abstract

Porphyromonas gingivalis is a Gram-negative, black pigmented oral anaerobeassociated with adult periodontitis. The adherence of the bacterium to junctional epithelial cells is the first step in infection and colonization. The molecular mechanisms and genetics of colonization are, as yet, not well understood, although it has been demonstrated that P. gingivalis fimbriae are involved in adhesion. In addition, cell surface cysteine proteinases mayplay a role either directly as adhesins or indirectly through their involvement in the biogenesis of fimbriae. A link has been established between cysteine proteinase-hemagglutinating activity and colongy pigmentation on blood agar. in this study a P. gingivalis ATCC 33277 transposon library was screened for white mutants. Pleiotropic mutants were identified with altered pigmentation, proteinase, hemagglutinin and haemolytic activities. Althoughthe mutants fell into two classes based on the above phenotypes, by electron microscopy both classes showed increased fimbriation and decreased vesicle formation. Sequencing of genomic DNA flanking the transposon insertions revealed that one class of mutants carried disruptions in the gene encodingLys-gingipain (kgp) and the other in a gene homologous to a glycosyl transferase. Potential roles for these genes in pigmentation, fimbriation, vesicle formation and attachment to epithelial cells are discussed. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:43:47