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Titolo:
Destabilization of tumor necrosis factor-alpha mRNA by 5-alpha dihydrotestosterone in Jurkat cells
Autore:
Takei, S; Redford, A; Katayama, S; Toyoda, H;
Indirizzi:
Univ Calif Los Angeles, Cedars Sinai Med Ctr, Sch Med, Dept Med,Div Med Genet, Los Angeles, CA 90048 USA Univ Calif Los Angeles Los Angeles CA USA 90048 Los Angeles, CA 90048 USA Saitama Med Sch, Dept Internal Med 4, Moroyama, Saitama 3500495, Japan Saitama Med Sch Moroyama Saitama Japan 3500495 ma, Saitama 3500495, Japan
Titolo Testata:
LIFE SCIENCES
fascicolo: 20, volume: 66, anno: 2000,
pagine: PL277 - PL282
SICI:
0024-3205(20000407)66:20<PL277:DOTNFM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; EXPRESSION; MOUSE; DIFFERENTIATION; PROGESTERONE; DISEASE; LINES; TNF; TH1;
Keywords:
TNF-alpha mRNA; mRNA stability; male steroid hormone; gene regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Toyoda, H Cedars Sinai Med Ctr, Dept Anat Pathol, 8700 Beverly Blvd, Los Angeles, CA90048 USA Cedars Sinai Med Ctr 8700 Beverly Blvd Los Angeles CA USA 90048
Citazione:
S. Takei et al., "Destabilization of tumor necrosis factor-alpha mRNA by 5-alpha dihydrotestosterone in Jurkat cells", LIFE SCI, 66(20), 2000, pp. PL277-PL282

Abstract

The effect of a male steroid hormone, 5DHT, on the expression of TNF-alphawas examined using a human leukemia T cell line, Jurkat. Cells were treated with 5DHT in the presence or absence of PHA, and RNA was isolated followed by a reverse transcriptase - mediated PCR (RT-PCR) to measure the steady state levels of TNF-alpha mRNA. The treatment of cells with 5DHT resulted in a 50% of decrease in the level of TNF-alpha mRNA compared to that in untreated conditions (basal level). A similar level of-reduction of the messageby 5DHT was also observed in PHA-stimulated cells. The reduction of the steady state levels of TNF-alpha mRNA in Jurkat cells was a result of destabilization of the gene as demonstrated by actinomycin D treatment; a half-life of TNF-alpha message in 5DHT treated cells and non-treated cells was 1 hrand 2.5 hr, respectively, whereas that in 5DHT/PHA and PHA-treated cells was 3hr and 6hr, respectively. (C) 2000 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 08:26:36