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Titolo:
Rabbit ventricular myocyte volume changes as a direct result of crystalloid cardioplegia in congestive heart failure induced by aortic regurgitation
Autore:
Danetz, JS; Davies, RD; Clemo, HF; Baumgarten, CM;
Indirizzi:
Virginia Commonwealth Univ, Med Coll Virginia, Dept Physiol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Med Coll Virginia, Dept Surg, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA Virginia Commonwealth Univ, Med Coll Virginia, Dept Internal Med, Div Cardiol, Richmond, VA 23298 USA Virginia Commonwealth Univ Richmond VA USA 23298 , Richmond, VA 23298 USA
Titolo Testata:
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
fascicolo: 4, volume: 119, anno: 2000,
parte:, 1
pagine: 826 - 833
SICI:
0022-5223(200004)119:4<826:RVMVCA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-VOLUME; CORONARY PERFUSION; PREVENTION; EDEMA; PROTECTION; RESISTANCE; ATRIAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Baumgarten, CM Virginia Commonwealth Univ, Med Coll Virginia, Dept Physiol, Box 980551, Richmond, VA 23298 USA Virginia Commonwealth Univ Box 980551 Richmond VA USA 23298
Citazione:
J.S. Danetz et al., "Rabbit ventricular myocyte volume changes as a direct result of crystalloid cardioplegia in congestive heart failure induced by aortic regurgitation", J THOR SURG, 119(4), 2000, pp. 826-833

Abstract

Objectives: We hypothesized that the cell volume of ventricular myocytes isolated from hearts in volume-overload congestive failure would respond differently to hypothermic cardioplegia than would sham-operated cohorts. Methods: Adult rabbits underwent either valvotomy and aortic regurgitation-induced heart failure or sham surgery, Congestive failure was confirmed clinically and by means of echocardiography. Cell volumes of isolated myocytes were measured by digital video microscopy. After equilibration in 37 degrees C physiologic solution, cells were suprafused with 9 degrees C standardor low-Cl- St Thomas' Hospital solution followed by reperfusion in 37 degrees C physiologic solution. Results: Exposure to cold St Thomas' Hospital solution for 20 minutes caused sham myocytes to swell by 8% (n = 9); cell volumes fully recovered on normothermic reperfusion. In contrast, congestive failure myocytes (n = 9) maintained their cell volume in cold St Thomas' Hospital solution and during reperfusion. Lowering the [K+][Cl-] product of SI Thomas' Hospital solutionby partially replacing Cl- with an impermeant anion prevented cellular edema in the sham group (n = 8) but caused a 4% swelling in failure myocytes (n = 10) on reperfusion. Osmotically shrinking the failure cells (n = 9) converted their behavior to that of sham cells. Conclusions: In the absence of ischermia, congestive failure myocytes are less sensitive to cardioplegia-induced edema than sham cells. Low-Cl- cardioplegia, which prevents edema and protects the normal heart, induced swelling and may be detrimental in myopathic hearts. Differences in volume regulation in failure and sham myocytes may be due to activation of volume-sensitive channels that are turned off by osmotic shrinkage.

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Documento generato il 20/09/20 alle ore 04:47:28