Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Antigenicity in soybean hypocotyls and its reduction by twin-screw extrusion
Autore:
Saitoh, S; Urushibata, M; Ikuta, K; Fujimaki, A; Harada, H;
Indirizzi:
Honen Corp, Food Res & Dev Labs, Shimizu, Shizuoka 4240824, Japan Honen Corp Shimizu Shizuoka Japan 4240824 himizu, Shizuoka 4240824, Japan
Titolo Testata:
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
fascicolo: 4, volume: 77, anno: 2000,
pagine: 419 - 424
SICI:
0003-021X(200004)77:4<419:AISHAI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALLERGENIC PROTEIN; IDENTIFICATION; ISOFLAVONES; SERA;
Keywords:
antigenicity; antigens; ELISA; embryo; hypocotyl; isoflavone; protein; trypsin inhibitor; twin-screw extrusion; urease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Saitoh, S Honen Corp, Food Res & Dev Labs, Shinminato 2, Shimizu, Shizuoka4240824, Japan Honen Corp Shinminato 2 Shimizu Shizuoka Japan 4240824 24, Japan
Citazione:
S. Saitoh et al., "Antigenicity in soybean hypocotyls and its reduction by twin-screw extrusion", J AM OIL CH, 77(4), 2000, pp. 419-424

Abstract

The purpose of the present study was to develop a simple method to make a low-antigenicity food and/or feed rich in isoflavones from soybean hypocotyls. The antigenicity of soybean hypocotyls for bovine antisoybean sera was assessed by enzyme-linked immunosorbent assay. Immunoblotting demonstrated that the antigenicity was derived from storage proteins, which were presentin hypocotyls as glycinin and beta-conglycinin, and from unknown proteins. Ground soybean hypocotyls (32-mesh sieve size) were passed through a twin-screw extruder to reduce the antigenicity to 1% of the original activity. The degradation of antigen proteins in soybean products was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Trypsin inhibitor and urease activity were also greatly reduced. The concentrations of isoflavones were unaffected.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:23:58