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Titolo:
Cathepsin B and its endogenous inhibitor cystatin C in rheumatoid arthritis synovium
Autore:
Hansen, T; Petrow, PK; Gaumann, A; Keyszer, GM; Eysel, P; Eckardt, A; Brauer, R; Kriegsmann, J;
Indirizzi:
Univ Mainz, Inst Pathol, D-55101 Mainz, Germany Univ Mainz Mainz GermanyD-55101 nz, Inst Pathol, D-55101 Mainz, Germany Univ Mainz, Clin Orthoped, D-55101 Mainz, Germany Univ Mainz Mainz Germany D-55101 , Clin Orthoped, D-55101 Mainz, Germany Univ Jena, Inst Pathol, D-6900 Jena, Germany Univ Jena Jena Germany D-6900 iv Jena, Inst Pathol, D-6900 Jena, Germany Univ Halle Wittenberg, Dept Internal Med 1, Halle, Germany Univ Halle Wittenberg Halle Germany Dept Internal Med 1, Halle, Germany
Titolo Testata:
JOURNAL OF RHEUMATOLOGY
fascicolo: 4, volume: 27, anno: 2000,
pagine: 859 - 865
SICI:
0315-162X(200004)27:4<859:CBAIEI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTEINE PROTEINASE-INHIBITOR; MESSENGER-RNA; RAT OSTEOCLASTS; GAMMA-TRACE; EXPRESSION; IDENTIFICATION; COLLAGENASE; DEGRADATION; TISSUES; BETA;
Keywords:
cathepsin B; cystatin C; rheumatoid arthritis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kriegsmann, J Univ Mainz, Inst Pathol, Langenbeckstr 1, D-55101 Mainz, Germany Univ Mainz Langenbeckstr 1 Mainz Germany D-55101 z, Germany
Citazione:
T. Hansen et al., "Cathepsin B and its endogenous inhibitor cystatin C in rheumatoid arthritis synovium", J RHEUMATOL, 27(4), 2000, pp. 859-865

Abstract

Objective. To compare the expression of cathepsin B and its endogenous inhibitor cystatin C in synovial tissue of patients with rheumatoid arthritis (RA) and to determine the cell type expressing cystatin C. Methods. The expression of cathepsin B and cystatin C was studied by immunohistochemistry in synovial tissue of 10 patients with RA and compared to healthy controls. Applying double labeling methods, the expression of cathepsin B was compared to that of cystatin C. To determine the cell type expressing cystatin C, double labeling with anti-CD68 (PG-M1) was performed. Results. Both cystatin C and cathepsin B were strongly expressed, in synoviocytes of patients with RA. Furthermore, fibroproliferative tissue at the:site of cartilage and bone destruction contained fibroblast-like and macrophage-like cells positive for cystatin C and cathepsin B, whereas normal synovial tissue exhibited only limited expression of these molecules. Osteoclasts revealed positive staining for CD68 and cystatin C, but not for cathepsin B. Conclusion. Cystatin C is a product of both macrophage-like and fibroblast-like synoviocytes. The strong expression of both the matrix degrading cysteine proteinase cathepsin B and the cysteine proteinase inhibitor cystatin C in rheumatoid synovium, particularly at the sites of bone and cartilage erosion, suggests that cystatin C - although increased - is not sufficient to prevent matrix degradation by cathepsin B.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 05:50:20