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Titolo:
Automated determination of reboxetine by high-performance liquid chromatography with column-switching and ultraviolet detection
Autore:
Hartter, S; Weigmann, H; Hiemke, C;
Indirizzi:
Univ Mainz, Dept Psychiat, D-55131 Mainz, Germany Univ Mainz Mainz Germany D-55131 , Dept Psychiat, D-55131 Mainz, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 740, anno: 2000,
pagine: 135 - 140
SICI:
1387-2273(20000331)740:1<135:ADORBH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-PLASMA; ANTIDEPRESSANTS; EFFICACY; DRUG; METABOLITES; FLUVOXAMINE; SERUM;
Keywords:
reboxetine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Hiemke, C Univ Mainz, Dept Psychiat, Untere Zahlbacher Str 8, D-55131 Mainz, Germany Univ Mainz Untere Zahlbacher Str 8 Mainz Germany D-55131 ermany
Citazione:
S. Hartter et al., "Automated determination of reboxetine by high-performance liquid chromatography with column-switching and ultraviolet detection", J CHROMAT B, 740(1), 2000, pp. 135-140

Abstract

A fully automated method including column-switching and isocratic high-performance Liquid chromatography (HPLC) was developed for quantitative analysis of the new antidepressant reboxetine, a noradrenaline reuptake inhibitor. After serum injection into the HPLC system and on-line sample clean-up ona silica C-8 (10x4.0 mm I.D.) clean-up column with an eluent consisting of2.5% acetonitrile in deionized water, the chromatographic separation was performed on an analytical column (Lichrospher CN; 250x4.6 mm I.D.) with an eluent of acetonitrile-aqueous potassium phosphate buffer (0.008 M, pH 6.4)(50:50). The UV detector was set at 273 or 226 nm. The limit of quantification was about 15 ng/ml at 273 nm and about 4 ng/ml at 226 nm. The day-to-day relative standard deviation ranged between 2.7 and 6.7% with recovery rates greater than or equal to 90%. Linear regression analyses revealed correlation coefficients >0.998. The method can be applied to therapeutic drug monitoring of reboxetine as well as pharmacokinetic studies. (C) 2000 Elsevier Science B. V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 13:00:22