Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential regulation of cadherins by dexamethasone in human osteoblastic cells
Autore:
Lecanda, F; Cheng, SL; Shin, CS; Davidson, MK; Warlow, P; Avioli, LV; Civitelli, R;
Indirizzi:
Washington Univ, Sch Med, Div Bone & Mineral Dis, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Mineral Dis, St Louis, MO 63110 USA Vanderbilt Univ, Sch Med, Div Dermatol, Nashville, TN 37232 USA VanderbiltUniv Nashville TN USA 37232 Dermatol, Nashville, TN 37232 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 3, volume: 77, anno: 2000,
pagine: 499 - 506
SICI:
0730-2312(2000)77:3<499:DROCBD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARROW STROMAL CELLS; ADHESION MOLECULE; EXPRESSION; MORPHOGENESIS; FAMILY;
Keywords:
osteoblast differentiation; cell-cell adhesion; cadherins; corticosteroids; hormonal regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Civitelli, R Barnes Jewish Hosp, Div Bone & Mineral Dis, 216 S Kingshighway Blvd, St Louis, MO 63110 USA Barnes Jewish Hosp 216 S Kingshighway Blvd St Louis MO USA 63110
Citazione:
F. Lecanda et al., "Differential regulation of cadherins by dexamethasone in human osteoblastic cells", J CELL BIOC, 77(3), 2000, pp. 499-506

Abstract

Human osteoblasts express a repertoire of cadherins, including N-cadherin (N-cad), cadherin-11 (C11), and cadherin-4 (C4). We have previously shown that direct cell-cell adhesion via cadherins is critical for BMP-2-induced osteoblast differentiation. in this study, we have analyzed the regulation of cadherin expression in normal human trabecular bone osteoblasts (HOB), and osteoprogenitor marrow stromal cells (BMC), during exposure to dexamethasone, another inducer of human bone cell differentiation. Dexamethasone inhibited the expression of both C11 and N-cad mRNA in both BMC and HOB, although the effect was much more pronounced on N-cad than on C11. This action ofthe steroid was dose dependent, was maximal at 10(-7) M concentration, andoccurred as early as after 1 day of incubation. By contrast, expression ofC4 mRNA and protein was strongly induced by dexamethasone in BMC and was stimulated in HOB. This stimulatory effect lasted for at least 2 weeks of incubation. A cadherin inhibitor, HAV-containing decapeptide only partially (similar to 50%) prevented dexamethasone-induced stimulation of alkaline phosphatase activity by BMC, which instead was not altered by incubation with a neutralizing antibody against C4. Therefore, the pattern of cadherin regulation by dexamethasone radically differs form that observed with BMP-2. Dexamethasone effects on certain osteoblast differentiated features, such as induction of alkaline phosphatase activity are not strictly dependent on cadherin function. J. Cell. Biochem. 77:499-506, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:29:16