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Titolo:
Influenza virus-induced NF-kappa B-dependent gene expression is mediated by overexpression of viral proteins and involves oxidative radicals and activation of I kappa B kinase
Autore:
Flory, E; Kunz, M; Scheller, C; Jassoy, C; Stauber, R; Rapp, UR; Ludwig, S;
Indirizzi:
Univ Wurzburg, Inst Med Strahlenkunde & Zellforsch, MSZ, D-97078 Wurzburg,Germany Univ Wurzburg Wurzburg Germany D-97078 ch, MSZ, D-97078 Wurzburg,Germany Univ Rostock, Dermatol & Venereol Klin, D-18055 Rostock, Germany Univ Rostock Rostock Germany D-18055 reol Klin, D-18055 Rostock, Germany Univ Wurzburg, Inst Virol & Immunbiol, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 unbiol, D-97078 Wurzburg, Germany Univ Erlangen Nurnberg, Inst Klin & Mol Virol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 12, volume: 275, anno: 2000,
pagine: 8307 - 8314
SICI:
0021-9258(20000324)275:12<8307:IVNBGE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; TRANSCRIPTIONAL REGULATION; RAF-1 KINASE; HBX PROTEIN; A VIRUSES; CELL; INFECTION; INDUCTION; INTERLEUKIN-8; SPECIFICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Ludwig, S Univ Wurzburg, Inst Med Strahlenkunde & Zellforsch, MSZ, Versbacherstr 5, D-97078 Wurzburg, Germany Univ Wurzburg Versbacherstr 5 WurzburgGermany D-97078 Germany
Citazione:
E. Flory et al., "Influenza virus-induced NF-kappa B-dependent gene expression is mediated by overexpression of viral proteins and involves oxidative radicals and activation of I kappa B kinase", J BIOL CHEM, 275(12), 2000, pp. 8307-8314

Abstract

Influenza A viruses are capable of inducing the expression of a variety ofcytokine and proapoptotic genes in infected cells. The promoter regions ofmost of these genes harbor binding sites for the transcription factor NF-kappa B which is an important mediator of immune and inflammatory responses. Our present study is based on an observation that influenza A virus infection of cells stimulates transcriptional activation of the HN-l long terminal repeat (LTR) which harbors two regulatory NF-kappa B elements, and is aimed at identifying the molecular mechanisms involved in this process. We found that the expression of influenza virus hemagglutinin (HA), matrix protein (RI), and nucleoprotein (NP), as single factors is sufficient to transcriptionally activate the HIV-1 LTR, This process is mediated by oxidative radicals because treatment of cells with pyrrolidine dithiocarbamate, a scavenger of such radicals, abolished the transactivating ability. Expression of different influenza proteins induces activation of NF-kappa B-dependent gene expression but not transcriptional activation of an AP-1/Ets-dependent promoter, indicating a selectivity for NF-kappa B transactivation, Furthermore, influenza protein expression induces activation of I kappa B kinase (IKK), Accordingly coexpression of a catalytically inactive mutant of IKK abolishes influenza protein induced activation of NF-kappa B as web as HIV-1 LTR-dependent reporter gene expression, suggesting that IKK is an important intermediate within this signaling process, Taken together, our results show that various influenza virus proteins act as viral transactivators to modulate transcriptional activity of kappa B-element harboring promoters such asthe HIV-LTR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:40:25