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Titolo:
Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme
Autore:
Papamichos-Chronakis, M; Conlan, RS; Gounalaki, N; Copf, T; Tzamarias, D;
Indirizzi:
Univ Crete, Inst Mol Biol & Biotechnol, Fdn Res & Technol, GR-71110 Iraklion, Crete, Greece Univ Crete Iraklion Crete Greece GR-71110 R-71110 Iraklion, Crete, Greece Univ Crete, Dept Biol, GR-71110 Iraklion, Crete, Greece Univ Crete Iraklion Crete Greece GR-71110 R-71110 Iraklion, Crete, Greece
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 12, volume: 275, anno: 2000,
pagine: 8397 - 8403
SICI:
0021-9258(20000324)275:12<8397:HIACCT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBOXY-TERMINAL DOMAIN; TRANSCRIPTIONAL REGULATION; SACCHAROMYCES-CEREVISIAE; YEAST; COMPLEX; REPRESSION; PROTEINS; ACTIVATION; RECRUITMENT; MEDIATOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Tzamarias, D Univ Crete, Inst Mol Biol & Biotechnol, Fdn Res & Technol, POB 1527,Vassilika Vouton, GR-71110 Iraklion, Crete, Greece Univ Crete POB 1527,Vassilika Vouton Iraklion Crete Greece GR-71110
Citazione:
M. Papamichos-Chronakis et al., "Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme", J BIOL CHEM, 275(12), 2000, pp. 8397-8403

Abstract

The Srb/Mediator, a multisubunit subcomplex of the RNA polymerase II (RNA pol II) holoenzyme has been proposed to function as a control panel regulating transcription in response to gene-specific activator proteins. In this report, we identify the Mediator subunit Hrs1/Med3 as a physical target forCyc8-Tup1, a yeast transcriptional corepressor. Two-hybrid and glutathioneS-transferase interaction assays show that Hrs1 can associate directly with Cyc8-Tup1, Moreover, affinity chromatography experiments, using yeast protein extracts, reveal that Cyc8-Tup1 co-purifies with Hrs1 and with additional Mediator subunits in a Hrs1 dependent manner. These observations suggest that Cyc8-Tup1 contacts the Mediator complex via its interaction with theHrs1 subunit, Further on, genetic analysis indicates that increased Hrs1 dosage can alleviate Cyc8-Tup1-mediated repression, suggesting that Hrs1/Mediator's function is inhibited upon its interaction with Cyc8-Tup1. Finally,artificial holoenzyme recruitment assays support a model by which the contact between the corepressor and the Hrs1/Mediator may prevent pol II holoenzyme recruitment to the core promoter. These data, together with previous genetic evidence, establish a functional and physical interaction between the Cyc8-Tup1 corepressor and the RNA pol II holoenzyme and support a centralrole of the Mediator complex in transcriptional repression.

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Documento generato il 19/09/20 alle ore 09:15:42