Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification and characterization of a novel inositol polyphosphate 5-phosphatase
Autore:
Ijuin, T; Mochizuki, Y; Fukami, K; Funaki, M; Asano, T; Takenawa, T;
Indirizzi:
Univ Tokyo, Inst Med Sci, Dept Biochem, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Biochem, Minato Ku, Tokyo 1088639, Japan Asahi Life Fdn, Inst Adult Dis, Shinjuku Ku, Tokyo 160, Japan Asahi Life Fdn Tokyo Japan 160 Adult Dis, Shinjuku Ku, Tokyo 160, Japan Univ Tokyo, Fac Med, Dept Internal Med 3, Bunkyo Ku, Tokyo 113, Japan UnivTokyo Tokyo Japan 113 t Internal Med 3, Bunkyo Ku, Tokyo 113, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 15, volume: 275, anno: 2000,
pagine: 10870 - 10875
SICI:
0021-9258(20000414)275:15<10870:IACOAN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 5-PHOSPHATASE; SIGNAL-TRANSDUCTION; HUMAN-PLATELETS; ALPHA-ACTININ; CLONING; POLYPHOSPHATE-5-PHOSPHATASE; 4,5-BISPHOSPHATE; SYNAPTOJANIN; EXPRESSION; 1,4,5-TRISPHOSPHATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Takenawa, T Univ Tokyo, Inst Med Sci, Dept Biochem, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 nato Ku, Tokyo 1088639, Japan
Citazione:
T. Ijuin et al., "Identification and characterization of a novel inositol polyphosphate 5-phosphatase", J BIOL CHEM, 275(15), 2000, pp. 10870-10875

Abstract

We have identified a cDNA encoding a novel inositol polyphosphate 5-phosphatase. It contains two highly conserved catalytic motifs for 5-phosphatase,has a molecular mass of 51 kDa, and is ubiquitously expressed and especially abundant in skeletal muscle, heart, and kidney. We designated this 5-phosphatase as SKIP (Skeletal muscle and Kidney enriched Inositol Phosphatase), SKIP is a simple 5-phosphatase with no other motifs. Baculovirus-expressed recombinant SKIP protein exhibited 5-phosphatase activities toward inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol (PtdIns) 4,5-bisphosphate, and PtdIns 3,4,5-trisphosphate but has 6-fold more substrate specificity for PtdIns 4,5-bisphosphate (K-m = 180 mu M)than for inositol 1,4,5-trisphosphate (K-m = 1.15 mM), The ectopic expression of SKIP protein in COS-7 cells and immunostaining of neuroblastoma N1E-115 cells revealed that SKIP is expressed in cytosol and that loss of actinstress fibers occurs where the SKIP protein is concentrated. These resultsimply that SKIP plays a negative role in regulating the actin cytoskeletonthrough hydrolyzing PtdIns 1,4-bisphosphate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:28:59