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Titolo:
Structural modeling and site-directed mutagenesis of the actinorhodin beta-ketoacyl-acyl carrier protein synthase
Autore:
He, M; Varoglu, M; Sherman, DH;
Indirizzi:
Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA Univ Minnesota, Biol Proc Technol Inst, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 l Inst, Minneapolis, MN 55455 USA
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 9, volume: 182, anno: 2000,
pagine: 2619 - 2623
SICI:
0021-9193(200005)182:9<2619:SMASMO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
STREPTOMYCES-COELICOLOR A3(2); ANTIBIOTICS; CERULENIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Sherman, DH Univ Minnesota, Dept Microbiol, Box 196,1460 Mayo Mem Bldg,420Delaware StSE, Minneapolis, MN 55455 USA Univ Minnesota Box 196,1460 Mayo Mem Bldg,420 Delaware St SE Minneapolis MN USA 55455
Citazione:
M. He et al., "Structural modeling and site-directed mutagenesis of the actinorhodin beta-ketoacyl-acyl carrier protein synthase", J BACT, 182(9), 2000, pp. 2619-2623

Abstract

A three-dimensional model of the Streptomyces coelicolor actinorhodin beta-ketoacyl synthase (Act KS) was constructed based on the X-ray crystal structure of the related Escherichia coli fatty acid synthase condensing enzymebeta-ketoacyl synthase II, revealing a similar catalytic active site organization in these two enzymes. The model was assessed by site-directed mutagenesis of five conserved amino acid residues in Act KS that are in close proximity to the Cys169 active site. Three substitutions completely abrogatedpolyketide biosynthesis, while two replacements resulted in significant reduction in polyketide production. H-3-cerulenin labeling of the various ActKS mutant proteins demonstrated that none of the amino acid replacements affected the formation of the active site nucleophile.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 01:55:17