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Titolo:
Cellular localization of fibroplast growth factor 2 (FGF-2) in benign prostatic hyperplasia
Autore:
Sinowatz, F; Schams, D; Einspanier, R; Arnold, G; Pfeffer, M; Temmim-Baker, L; Amselgruber, W; Plendl, J;
Indirizzi:
Univ Munich, Fac Vet, Inst Anat 2, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 Inst Anat 2, D-80539 Munich, Germany Tech Univ Munich, Inst Physiol, D-8050 Freising, Germany Tech Univ MunichFreising Germany D-8050 ysiol, D-8050 Freising, Germany Univ Munich, Genzentrum, Mol Biol Lab, D-8033 Martinsried, Germany Univ Munich Martinsried Germany D-8033 Lab, D-8033 Martinsried, Germany Univ Munich, Inst Med Microbiol Infect & Epidem Dis, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 & Epidem Dis, D-80539 Munich, Germany Kuwait Canc Control Ctr, Shuwaikh, Kuwait Kuwait Canc Control Ctr Shuwaikh Kuwait c Control Ctr, Shuwaikh, Kuwait
Titolo Testata:
HISTOLOGY AND HISTOPATHOLOGY
fascicolo: 2, volume: 15, anno: 2000,
pagine: 475 - 481
SICI:
0213-3911(200004)15:2<475:CLOFGF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
REGIONAL DISTRIBUTION; MUSCLE CELLS; FIBROBLAST; EXPRESSION; TISSUE; CARCINOMA; HORMONES; ADULT;
Keywords:
BPH; FGF-2; immunocytochemistry; in situ hybridisation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Sinowatz, F Univ Munich, Inst Vet Anat, Vet Str 13, D-80539 Munich, Germany Univ Munich Vet Str 13 Munich Germany D-80539 Munich, Germany
Citazione:
F. Sinowatz et al., "Cellular localization of fibroplast growth factor 2 (FGF-2) in benign prostatic hyperplasia", HIST HISTOP, 15(2), 2000, pp. 475-481

Abstract

Fibroblast growth factor 2 (FGF-2, basic fibroblast growth factor) has been reported to be elevated in tissues from benign prostatic hyperplasia (BPH), the most frequent neoplastic disease in aging men. This suggests that FGF-2 may play a significant role in the development of BPH. In this study the cellular distribution pattern of FGF-2 in tissues from BPH has been investigated by immunohistochemical and molecular biological methods. Radioimmunoassay revealed high concentrations of FGF-2, ranging between 450 and 950 ng per g tissue. Immunoblots confirmed the presence of a 18 kDa FGF-2 in tissue extracts. By immunohistochemistry done with a polyclonal antibody to recombinant FGF-2 on paraffin sections, FGF-2 was localized in fibroblasts, endothelial cells and smooth muscle cells of tissue samples of BPH. Nuclei of these cells were labelled distinctly. Moreover the cytoplasm of smooth muscle cells was labelled moderately. No immunostaining was seen in prostaticepithelium. Nonradioactive in situ hybridization with digoxygenin-labelledoligonucleotides revealed the presence of mRNA for FGF-2 in smooth muscle cells of the prostatic stroma. These results provide evidence that FGF-2 may be produced locally in the human prostate as a stroma-specific mitogen and may play a causal role in the development of BPH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:24:39