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Titolo:
Human uterine myocytes retain their energy charge with no gross alterations in morphology for at least 8 days when cultured under anaerobic conditions
Autore:
Olovsson, M; Nordling, M; Ulmsten, U; Lindblom, B; Waldenstrom, A; Ronquist, G;
Indirizzi:
Uppsala Univ, Dept Womens & Childrens Hlth, Sect Obstet & Gynecol, SE-75185 Uppsala, Sweden Uppsala Univ Uppsala Sweden SE-75185 & Gynecol, SE-75185 Uppsala, Sweden Uppsala Univ, Dept Clin Chem, SE-75185 Uppsala, Sweden Uppsala Univ Uppsala Sweden SE-75185 Clin Chem, SE-75185 Uppsala, Sweden Univ Umea Hosp, Dept Internal Med, S-90185 Umea, Sweden Univ Umea Hosp Umea Sweden S-90185 pt Internal Med, S-90185 Umea, Sweden
Titolo Testata:
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
fascicolo: 3, volume: 49, anno: 2000,
pagine: 165 - 169
SICI:
0378-7346(2000)49:3<165:HUMRTE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREGNANT HUMAN UTERUS; VASCULAR SMOOTH-MUSCLE; AEROBIC GLYCOLYSIS; IN-VITRO; METABOLISM; TERM; ACTIVATION; TRANSPORT; ECONOMY; TAENIA;
Keywords:
myocytes; cell culture; energy charge;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Olovsson, M Uppsala Univ, Dept Womens & Childrens Hlth, Sect Obstet & Gynecol, SE-75185 Uppsala, Sweden Uppsala Univ Uppsala Sweden SE-75185 E-75185 Uppsala, Sweden
Citazione:
M. Olovsson et al., "Human uterine myocytes retain their energy charge with no gross alterations in morphology for at least 8 days when cultured under anaerobic conditions", GYNECOL OBS, 49(3), 2000, pp. 165-169

Abstract

Objective: To investigate the ability of human uterine myocytes to grow under anaerobic conditions for a prolonged time period. Methods: Cells were isolated from fundal myometrium and cultured until subconfluency. The cell type was confirmed by immunostaining for the smooth muscle cell-specific cytoskeletal proteins alpha-actin and desmin, Some cells were further culturedunder aerobic conditions and others under anaerobic conditions. Cells wereharvested after 0, 4 and 8 days in culture and analyzed for their content of adenylates. Results: Immunostaining revealed that all three preparationscontained almost only smooth muscle cells. Energy charge of the myocytes was 0.88 on average at the beginning of the culture experiment. A moderate decrease was noted on day 4 for myocytes grown under both aerobic and anaerobic conditions and no further decrease was noted between days 4 and 8. Morphologically the cells retained their normal appearance and they seemed healthy for at least 8 days in culture under both aerobic and anaerobic conditions. Conclusions: The results of this study suggest that human myometrial cells can survive for an extended period of time under in vitro conditions regardless of oxygen availability for energy metabolism. This means that anaerobic energy metabolism is enough to sustain vital processes during that period of time. Copyright (C) 2000 S. Karger AG, Baser.

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Documento generato il 04/12/20 alle ore 16:40:54