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Biodiversity of Asian rice gall midge (Orseolia oryzae Wood Mason) from five countries examined by AFLP analysis
Katiyar, SK; Chandel, G; Tan, Y; Zhang, Y; Huang, B; Nugaliyadde, L; Fernando, K; Bentur, JS; Inthavong, S; Constantino, S; Bennett, J;
Int Rice Res Inst, Div Plant Breeding Genet & Biochem, Los Banos, Laguna, Philippines Int Rice Res Inst Los Banos Laguna Philippines anos, Laguna, Philippines Indira Gandhi Agr Univ, Raipur, Madhya Pradesh, India Indira Gandhi Agr Univ Raipur Madhya Pradesh India Madhya Pradesh, India Guangdong Acad Agr Sci, Inst Plant Protect, Guangzhou, Peoples R China Guangdong Acad Agr Sci Guangzhou Peoples R China gzhou, Peoples R China Rice Genet & Dev Inst, Batalagoda, Sri Lanka Rice Genet & Dev Inst Batalagoda Sri Lanka Inst, Batalagoda, Sri Lanka Plant Genet Resources Ctr, Peredeniya, Sri Lanka Plant Genet Resources Ctr Peredeniya Sri Lanka r, Peredeniya, Sri Lanka Directorate Rice Res, Hyderabad, Andhra Pradesh, India Directorate Rice Res Hyderabad Andhra Pradesh India ndhra Pradesh, India Natl Agr Res Project, Vientiane, Laos Natl Agr Res Project Vientiane Laos tl Agr Res Project, Vientiane, Laos
Titolo Testata:
fascicolo: 2, volume: 43, anno: 2000,
pagine: 322 - 332
biotypes; Cecidomyiidae; insect; Oryza sativa; Platygaster oryzae; population; sexual dimorphism;
Tipo documento:
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Indirizzi per estratti:
Indirizzo: Bennett, J Int Rice Res Inst, Div Plant Breeding Genet & Biochem, Los Banos, Laguna, Philippines Int Rice Res Inst Los Banos Laguna Philippines a, Philippines
S.K. Katiyar et al., "Biodiversity of Asian rice gall midge (Orseolia oryzae Wood Mason) from five countries examined by AFLP analysis", GENOME, 43(2), 2000, pp. 322-332


Amplified fragment length polymorphism (AFLP) analysis was used to assess the biodiversity of one of the most important dipteran pests of cereals, the Asian rice gall midge (Orseolia oryzae Wood Mason). Larvae and pupae werecollected at 15 locations in five Asian countries and preserved in 95% ethanol for storage, shipment, and DNA extraction using cetyltrimethylammoniumbromide (CTAB). Although only similar to 1 mu g of DNA was extracted from a single pupa or larva, the use of several AFLP primers in various combinations meant that this amount of DNA was sufficient to allow many DNA fingerprints to be made per individual. Fingerprints were sufficiently reproducible, especially during selective amplification, to allow the genetic diversity within a field population to be characterized. Extraction of DNA from a pool of 20 insects yielded AFLP fingerprints in which variation among individuals was sacrificed in favor of detecting differences among populations. For each location, pooled DNA was amplified with three primer pairs. A totalof 261 distinct AFLP bands were identified for the 45 fingerprints. Cluster analysis, performed by the unweighted pair-group method (UPGMA), separated the populations into two distinct groups. Group I included two populations from Guangdong province of southern China and one each from Laos and Imphal in northeastern India, while group II was comprised of eleven populations from elsewhere in India (Assam, Orissa, Madhya Pradesh, Andhra Pradesh, and Kerala) and from Nepal and Sri Lanka. AFLP analysis provided insight into the origins of gall midge biotypes. In 1992, the prevailing biotype in Imphal changed from Indian biotype 3 to a new biotype 3M. Our data show that biotype 3M belongs to group I and did not arise by a recent mutation from biotype 3, which belongs to group II. By contrast, Indian biotypes 2 and 4 are likely to have diverged through recent mutation and selection, as are Chinese biotypes 1 and 4. The almost simultaneous emergence of new biotypes in Kerala and Sri Lanka during 1985-1988 was most probably coincidental, because these biotypes are not closely related. AFLP fingerprints were also able to detect sexual dimorphism in the DNA of adult gall midges and to distinguish gall midge from its major parasite Platygaster oryzae.

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Documento generato il 18/01/21 alle ore 16:22:02