Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The Drosophila HEM-2/NAP1 homolog KETTE controls axonal pathfinding and cytoskeletal organization
Autore:
Hummel, T; Leifker, K; Klambt, C;
Indirizzi:
Univ Munster, Inst Neurobiol, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 Neurobiol, D-48149 Munster, Germany
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 7, volume: 14, anno: 2000,
pagine: 863 - 873
SICI:
0890-9369(20000401)14:7<863:TDHHKC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH CONE GUIDANCE; BINDING PROTEIN RAC; CELL-SHAPE CHANGES; ACTIN-DEPENDENT PROCESSES; ALDRICH SYNDROME PROTEIN; SH2/SH3 ADAPTER PROTEIN; MIDLINE GLIAL-CELLS; GENETIC-ANALYSIS; XENOPUS-LAEVIS; STRESS FIBERS;
Keywords:
Drosophila; kette; PAK; axon guidance; HEM-2/NAP1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Klambt, C Univ Munster, Inst Neurobiol, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 D-48149 Munster, Germany
Citazione:
T. Hummel et al., "The Drosophila HEM-2/NAP1 homolog KETTE controls axonal pathfinding and cytoskeletal organization", GENE DEV, 14(7), 2000, pp. 863-873

Abstract

In Drosophila, the correct formation of the segmental commissures depends on neuron-glial interactions at the midline. The VUM midline neurons extendaxons along which glial cells migrate in between anterior and posterior commissures. Here, we show that the gene kette is required for the normal projection of the VUM axons and subsequently disrupts glial migration. Axonal projection defects are also found for many other moto- and interneurons. Inaddition, kette affects the cell morphology of mesodermal and epidermal derivatives, which show an abnormal actin cytoskeleton. The KETTE protein is homologous to the transmembrane protein HEM-2/NAP1 evolutionary conserved from worms to vertebrates. In vitro analysis has shown a specific interaction of the vertebrate HEM-2/NAP1 with the SH2-SH3 adapter protein NCK and thesmall GTPase RAC1, which both have been implicated in regulating cytoskeleton organization and axonal growth. Hypomorphic kette mutations lead to axonal defects similar to mutations in the Drosophila NCK homolog dreadlocks. Furthermore, we show that kette and dock mutants genetically interact. NCK is thought to interact with the small G proteins RAC1 and CDC42, which playa role in axonal growth. In line with these observations, a kette phenocopy can be obtained following directed expression of mutant DCDC42 or DRAC1 in the CNS midline. In addition, the kette mutant phenotype can be partiallyrescued by expression of an activated DRAC1 transgene. Our data suggest animportant role of the HEM-2 protein in cytoskeletal organization during axonal pathfinding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:32:37