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Titolo:
CD59 cross-linking induces secretion of APO2 ligand in overactivated humanT cells
Autore:
Monleon, I; Martiinez-Lorenzo, MJ; Anel, A; Lasierra, P; Larrad, L; Pineiro, A; Naval, J; Alava, MA;
Indirizzi:
Univ Zaragoza, Fac Ciencias, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 & Celular, E-50009 Zaragoza, Spain Univ Zaragoza, Hosp Clin Univ, Serv Inmunol, Zaragoza, Spain Univ Zaragoza Zaragoza Spain p Clin Univ, Serv Inmunol, Zaragoza, Spain
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 4, volume: 30, anno: 2000,
pagine: 1078 - 1087
SICI:
0014-2980(200004)30:4<1078:CCISOA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATION-INDUCED DEATH; INSOLUBLE MEMBRANE DOMAINS; TUMOR-NECROSIS-FACTOR; FAS-LIGAND; TYROSINE PHOSPHORYLATION; SIGNAL-TRANSDUCTION; APOPTOSIS; RECEPTOR; INDUCTION; COMPLEX;
Keywords:
T lymphocyte; activation-induced cell death; CD59; Fas/FasL; APO2 ligand; TRAIL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Anel, A Univ Zaragoza, Fac Ciencias, Dept Bioquim & Biol Mol & Celular, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 ar, E-50009 Zaragoza, Spain
Citazione:
I. Monleon et al., "CD59 cross-linking induces secretion of APO2 ligand in overactivated humanT cells", EUR J IMMUN, 30(4), 2000, pp. 1078-1087

Abstract

Jurkat cells and the derived TCR/CD3-defective subline, J.RT3.T3.5 undergoactivation-induced cell death (AICD) when stimulated with phytohemagglutinin (PHA). Since J.RT3.T3.5 cells do not express antigen receptor, we searched for the molecules that could be ligated by PHA and induce AICD in this cell line. We show here that the glycosyiphosphatidylinositol-linked CD59 molecule is expressed at the surface of Jurkat and J.RT3.T3.5 cells, and whencross-linked by specific antibodies can induce cell death. The toxicity ofsupernatants from PHA-stimulated Jurkat or J.RT3.T3.5 cells was prevented by a combination of the blocking anti-fas mAb SM1/23 and anti-APO2L/TRAIL mAb 5C2. However, toxicity of supernatants from anti-CD59 stimulated cells was specifically prevented by the anti-APO2L blocking antibody. Anti-CD59 cross-linking induced AICD also in normal human T cell blasts, which secretedtoxic molecules into the supernatant. The toxicity of these supernatants on Jurkat cells was fully prevented by the anti-APO2L blocking antibody, showing that CD59 crosslinking induces the preferential release of APO2L also in normal T cells. The possible physiological and/or pathological consequences of this observation are discussed.

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Documento generato il 20/10/20 alle ore 22:08:14