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Titolo:
Culture of bone marrow cells in GM-CSF plus high doses of lipopolysaccharide generates exclusively immature dendritic cells which induce alloantigen-specific CD4 T cell anergy in vitro
Autore:
Lutz, MB; Kukutsch, NA; Menges, M; Rossner, S; Schuler, G;
Indirizzi:
Univ Erlangen Nurnberg, Dept Dermatol, D-91052 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91052 -91052 Erlangen, Germany
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 4, volume: 30, anno: 2000,
pagine: 1048 - 1052
SICI:
0014-2980(200004)30:4<1048:COBMCI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; IN-VIVO; FACTOR-ALPHA; IFN-GAMMA; PROLIFERATION; DEACTIVATION; MATURATION; CYTOKINES;
Keywords:
dendritic cell; anergy; lipopolysaccharide; maturation; endotoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Lutz, MB Univ Erlangen Nurnberg, Dept Dermatol, Hartmannstr 14, D-91052 Erlangen, Germany Univ Erlangen Nurnberg Hartmannstr 14 Erlangen Germany D-91052 y
Citazione:
M.B. Lutz et al., "Culture of bone marrow cells in GM-CSF plus high doses of lipopolysaccharide generates exclusively immature dendritic cells which induce alloantigen-specific CD4 T cell anergy in vitro", EUR J IMMUN, 30(4), 2000, pp. 1048-1052

Abstract

Dendritic cells (DC) can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Bacterial stimuli such as endotoxin/lipopolysaccharide (LPS) can induce their final maturation. When BM-DC cultures were treated at day 6 or later with LPS, this final maturation was induced in vitro within 24 h. Such mature DC exhibited high levels of surface MHC II molecules and potent T cell sensitizing, but reduced endocytosis capacity. In contrast, immature DC express onlyfew MHC II molecules and are weak T cell stimulators but highly endocytic. When BM-DC cultures in GM-CSF were treated with 1 mu g/ml LPS at day 0 of the culture or throughout the culture, only immature DC developed as defined by phenotype (MHC II low) and function (high endocytosis, weak primary mixed lymphocyte reaction). Those early LPS-treated immature DC induced alloantigen-specific anergy of CD4(+) T cells in vitro. These findings might contribute to the understanding of reduced T cell immunity in the course of septic shock and find application in DC-mediated tolerogenic immunotherapy strategies.

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Documento generato il 28/11/20 alle ore 21:30:14