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Titolo:
Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.)
Autore:
Chang, YJ; Barker, RE; Reed, BM;
Indirizzi:
USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA USDA ARS Corvallis OR USA 97333 plasm Repository, Corvallis, OR 97333 USA USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA USDA ARSCorvallis OR USA 97331 eed Prod Res Ctr, Corvallis, OR 97331 USA Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA Oregon State Univ Corvallis OR USA 97331 pt Hort, Corvallis, OR 97331 USA
Titolo Testata:
CRYO-LETTERS
fascicolo: 2, volume: 21, anno: 2000,
pagine: 107 - 116
SICI:
0143-2044(200003/04)21:2<107:CAIROC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSEQUENT PLANT-REGENERATION; SHOOT TIPS; MERISTEMS; CELLS; VITRIFICATION; SURVIVAL; DEHYDRATION;
Keywords:
cold acclimation; dehydration tolerance; ryegrass; genetic resources; liquid nitrogen; meristem; zoysiagrass;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Reed, BM USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA USDA ARS Corvallis OR USA 97333 ository, Corvallis, OR 97333 USA
Citazione:
Y.J. Chang et al., "Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.)", CRYO-LETT, 21(2), 2000, pp. 107-116

Abstract

Cold acclimation of Lolium L. and Zoysia Willd. grass cultivars significantly increased regrowth of cryopreserved meristems. One wk of cold acclimation improved recovery following cryopreservation but extended cold acclimation (4-8 wk) resulted in the best regrowth. Cold acclimation also significantly increased the dehydration tolerance of both Zoysia and Lolium meristems. Lolium apices cold acclimated for 4 wk produced 60-100% regrowth following cryopreservation by slow freezing or encapsulation-dehydration. Cold-acclimated Zoysia had greater than 60% regrowth following encapsulation-dehydration when beads were dehydrated to less than 22% water content. Non-acclimated meristems of both genera had little or no regrowth. Thawed meristems grew quickly without callus formation and the plantlets produced were transplanted to pots in the greenhouse after 4 to 6 wk. Samples of each cultivar were stored in liquid nitrogen as part of the U.S. National Plant Germplasm System.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:34:40