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Titolo:
UV resonance Raman study of angiotensin II conformation in nonaqueous environments: Lipid micelles and acetonitrile
Autore:
Holtz, JSW; Lednev, IK; Asher, SA;
Indirizzi:
Univ Pittsburgh, Chevron Sci Ctr, Dept Chem, Pittsburgh, PA 15260 USA UnivPittsburgh Pittsburgh PA USA 15260 pt Chem, Pittsburgh, PA 15260 USA
Titolo Testata:
BIOPOLYMERS
fascicolo: 2, volume: 57, anno: 2000,
pagine: 55 - 63
SICI:
0006-3525(2000)57:2<55:URRSOA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN SECONDARY STRUCTURE; SIDE-CHAIN CONFORMATION; EXCITATION PROFILES; CIRCULAR-DICHROISM; PEPTIDE-BOND; LASER SOURCE; ANALOGS; SPECTROSCOPY; TYROSINE; DOMAIN;
Keywords:
UV resonance Raman; angiotensin II; conformation; nonaqueous enviroments; lipid micelles; acetonitrile;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Asher, SA Univ Pittsburgh, Chevron Sci Ctr, Dept Chem, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 ittsburgh, PA 15260 USA
Citazione:
J.S.W. Holtz et al., "UV resonance Raman study of angiotensin II conformation in nonaqueous environments: Lipid micelles and acetonitrile", BIOPOLYMERS, 57(2), 2000, pp. 55-63

Abstract

We used 206.5-nm excited resonance Raman measurements to examine the angiotensin II (AII) secondary structure in H2O in the presence of dodecylphosphocholine (DPC) micelles, sodium dodecylsulfate (SDS) monomers and micelles,and in a 70% acetonitrile (ACN-d)-30% water solution. Our AII-SDS titration absorption studies indicate the formation of a 1:2 AII:SDS complex in which two negatively charged SDS molecules attach to the AII positively charged N terminus and to Arg(2). Our 206.5-nm excited Raman results indicate that the 1:2 AII:SDS complexation increases the AII beta-turn composition. We also used 228.9-nm Raman excitation to probe the local solvent accessibility of Tyr(4) (AII) in DPC and SDS micelles. Our Tyr (AII) solvent accessibility studies suggest that the Tyr residue is more exposed to the aqueous environment in SDS micelles than in DPC micelles. (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:29:59