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Titolo:
Effects of progestins and relaxin on glycodelin gene expression in human endometrial cells
Autore:
Taylor, RN; Vigne, JL; Zhang, PL; Hoang, P; Lebovic, DI; Mueller, MD;
Indirizzi:
Univ Calif San Francisco, Sch Med, Dept Obstet Gynecol & Reprod Sci, Ctr Reprod Endocrinol, San Francisco, CA 94143 USA Univ Calif San Francisco SanFrancisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
fascicolo: 4, volume: 182, anno: 2000,
pagine: 841 - 847
SICI:
0002-9378(200004)182:4<841:EOPARO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLACENTAL PROTEIN-14; MENSTRUAL-CYCLE; IN-VITRO; PREGNANCY; FERTILIZATION; IMPLANTATION; PROGESTERONE; SECRETION; MARKER;
Keywords:
glycodelin; implantation; placental protein 14; uterus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Taylor, RN Univ Calif San Francisco, Sch Med, Dept Obstet Gynecol & ReprodSci, Ctr Reprod Endocrinol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 94143 USA
Citazione:
R.N. Taylor et al., "Effects of progestins and relaxin on glycodelin gene expression in human endometrial cells", AM J OBST G, 182(4), 2000, pp. 841-847

Abstract

OBJECTIVE: Glycodelin is an endometrial protein proposed to play an important role in embryonic implantation. We examined the effects of progestins and relaxin on glycodelin transcription, synthesis, and secretion. STUDY DESIGN: Northern blotting, metabolic labeling, and fluorography wereused to assess glycodelin messenger ribonucleic acid and protein synthesisin endometrial tissue and cells. Luciferase reporter constructs transfected into endometrial adenocarcinoma cells (Ishikawa cells) were used to determine whether progestins or relaxin could activate the glycodelin gene promoter. RESULTS: Progestins but not relaxin stimulated glycodelin secretion in primary epithelial cell cultures. A 452-base pair fragment of the glycodelin gene promoter was activated 4.3 +/- 0.7 times normal by 10-nmol/L promegestone; however, addition of relaxin to the same construct repressed progestin-stimulated promoter activation by >30%. CONCLUSION: Glycodelin transcription, synthesis, and secretion by endometrial epithelial cells were stimulated by progestins. However, relaxin failedto stimulate production of this immunomodulatory protein and, in fact, repressed progestin-stimulated activation of the glycodelin gene promoter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:54:19