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Titolo:
Differential effect of TPA on cell growth and Epstein-Barr virus reactivation in epithelial cell lines derived from gastric tissues and B cell line Raji
Autore:
Kanamori, M; Tajima, M; Satoh, Y; Hoshikawa, Y; Miyazawa, Y; Okinaga, K; Kurata, T; Sairenji, T;
Indirizzi:
Tottori Univ, Fac Med, Sch Life Sci, Dept Biosignaling, Yonago, Tottori 6838503, Japan Tottori Univ Yonago Tottori Japan 6838503 Yonago, Tottori 6838503, Japan Teikyo Univ, Sch Med, Dept Clin Pathol, Cent Clin Lab, Tokyo 1730003, Japan Teikyo Univ Tokyo Japan 1730003 hol, Cent Clin Lab, Tokyo 1730003, Japan Teikyo Univ, Sch Med, Dept Surg, Tokyo 1730003, Japan Teikyo Univ Tokyo Japan 1730003 Sch Med, Dept Surg, Tokyo 1730003, Japan Natl Inst Infect Dis, Dept Pathol, Tokyo 1620052, Japan Natl Inst Infect Dis Tokyo Japan 1620052 pt Pathol, Tokyo 1620052, Japan
Titolo Testata:
VIRUS GENES
fascicolo: 2, volume: 20, anno: 2000,
pagine: 117 - 125
SICI:
0920-8569(200004)20:2<117:DEOTOC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; PROMOTING PHORBOL ESTERS; PRODUCTIVE INFECTION; LYTIC CYCLE; CARCINOMA; INDUCTION; LATENCY; GENOME; IDENTIFICATION; TRANSFORMATION;
Keywords:
Epstein-Barr virus (EBV); EBV infected cell lines; EBV reactivation; cell growth; cell cycle; TPA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sairenji, T Tottori Univ, Fac Med, Sch Life Sci, Dept Biosignaling, Yonago, Tottori 6838503, Japan Tottori Univ Yonago Tottori Japan 6838503 tori 6838503, Japan
Citazione:
M. Kanamori et al., "Differential effect of TPA on cell growth and Epstein-Barr virus reactivation in epithelial cell lines derived from gastric tissues and B cell line Raji", VIRUS GENES, 20(2), 2000, pp. 117-125

Abstract

We characterized the cell growth and Epstein-Barr virus (EBV) reactivationfor EBV infected epithelial cell lines, GT38, GT39, and GTC-4 using 12-O-tetradecanoylphorbol-13-acetate (TPA). These cell lines grew similarly in liquid medium, and formed colonies in soft agar. The cell growth was inhibited with TPA, dose-dependently in liquid medium. The colony formation was enhanced with low concentrations of TPA, but was inhibited with high concentrations. The latent EBV was reactivated with high concentrations of TPA as shown by the expression of EBV BZLF1 gene product ZEBRA. The effects of TPA on GTC-4 were compared with a Burkitt's lymphoma cell line Raji. The mode ofactions of TPA in GTC-4 was different from Raji in terms of cell growth and EBV reactivation. The effective concentrations of TPA for cell growth inhibition and EBV reactivation were higher in Raji than GTC-4. Cell cycle analysis showed that TPA (20 ng/ml) induced cell cycle arrest to Raji but not to GTC-4; however, the rate of trypan blue stained cells increased in the TPA treated GTC-4 but not Raji. These results demonstrated that TPA affects differentially for the stimulation and inhibition of cell growth, and also EBV reactivation depends on TPA concentrations and cell types.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:18:08