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Titolo:
Cytokine mRNA expression in leukocytes of efferent lymph from stimulated lymph nodes in pigs
Autore:
Reddy, J; Borgs, P; Wilkie, BN;
Indirizzi:
Univ Guelph, Dept Pathobiol, Guelph, ON NIG 2WI, Canada Univ Guelph Guelph ON Canada NIG 2WI athobiol, Guelph, ON NIG 2WI, Canada
Titolo Testata:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
fascicolo: 1-2, volume: 74, anno: 2000,
pagine: 31 - 46
SICI:
0165-2427(20000419)74:1-2<31:CMEILO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; LYMPHOTOXIN-ALPHA; IMMUNE-RESPONSE; MESSENGER-RNA; T-CELLS; SKIN; DIFFERENTIATION; INTERLEUKIN-12; PROLIFERATION; TRANSCRIPTION;
Keywords:
porcine immune response; cytokines; lymph node; quantitative reverse transcription polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Wilkie, BN Univ Guelph, Dept Pathobiol, Guelph, ON NIG 2WI, Canada Univ Guelph Guelph ON Canada NIG 2WI uelph, ON NIG 2WI, Canada
Citazione:
J. Reddy et al., "Cytokine mRNA expression in leukocytes of efferent lymph from stimulated lymph nodes in pigs", VET IMMUNOL, 74(1-2), 2000, pp. 31-46

Abstract

To test the hypothesis that characteristic cytokine responses occur in stimulated porcine lymph nodes (LNs), lymph node efferent ducts were surgically cannulated. Efferent lymph (EL) leukocytes were collected before and after stimulation of LNs with mitogens [bacterial lipopolysaccharide (LPS) or phytohemagglutinin-P(PHA-P)] and antigens [hen egg white lysozyme (HEWL) or purified protein derivative of tuberculin (PPD)]. Cytokine mRNA expression was evaluated by quantitative reverse transcription polymerase chain reaction (Q-RT-PCR). Interleukin (IL)-1 alpha was predominantly produced after all stimuli except for HEWL after which tumour necrosis factor (TNF)-alpha message was dominant. None of the stimuli induced message for IL-2, IL-4 or IL-8. Other cytokine mRNAs were produced in variable amounts and percentage of overall production of each cytokine message was in the following descending rank: LPS: IL-1 alpha, TNF-alpha, interferon (IFN)-gamma, IL-10, IL-12-p35, IL-6, IL-12-p40 and TNF-beta; PHA-P: IL-1 alpha, TNF-alpha, IL-10, IFN-gamma, IL-12-p40 and TNF-beta; HEWL: TNF-alpha, IL-1 alpha, IFN-gamma, IL-10, IL-6, IL-12-p40, TNF-beta and IL-12-p35 and PPD: IL-1 alpha, IFN-gamma,TNF-alpha and IL-10. Time course response of cytokines revealed early (IL-1 alpha, 10, TNF-alpha) and intermediate (IL-12-p40, TNF-beta, IFN-gamma) responses for PHA-P and early (IL-1 alpha, 6, 10, IL-12-p35, IL-12-p40, TNF-alpha), intermediate (TNF-beta, IFN-gamma) and late (IL-1 alpha, 6) for LPS. Cytokine mRNA response induced by HEWL was early (IL-alpha, IFN-gamma), intermediate (IL-10, IL-12-p40, TNF-beta), late (IL-1 alpha, IL-12-p35) and very late (IL-1 alpha, 6, 10, IL-12-p40, TNF-alpha). In Bacillus Calmette-Guerin (BCG) sensitized pigs, stimulation of LNs with PPD induced message for IL-1 alpha, 10, TNF-alpha and IFN-gamma which peaked at 24 h. Cytokine mRNAs varied by stimulus and differed for antibody and cell-mediated immune response. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 20:02:03