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Titolo:
A new ABC transporter mediating the detachment of lipid-modified proteins from membranes
Autore:
Yakushi, T; Masuda, K; Narita, S; Matsuyama, S; Tokuda, H;
Indirizzi:
Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan Univ Tokyo Tokyo Japan 1130032 r Biosci, Bunkyo Ku, Tokyo 1130032, Japan
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 4, volume: 2, anno: 2000,
pagine: 212 - 218
SICI:
1465-7392(200004)2:4<212:ANATMT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI LIPOPROTEINS; OUTER-MEMBRANE; LOCALIZATION; BACTERIA; LOLA; SEQUENCES; SUBUNITS; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Tokuda, H Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1130032, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1130032 Tokyo 1130032, Japan
Citazione:
T. Yakushi et al., "A new ABC transporter mediating the detachment of lipid-modified proteins from membranes", NAT CELL BI, 2(4), 2000, pp. 212-218

Abstract

Lipoproteins in Escherichia coli are anchored to the periplasmic side of either the inner or the outer membrane by a lipid moiety that is covalently attached to the amino-terminal cysteine residue, Membrane specificity depends on a sorting signal at position 2 of the lipoprotein. Lipoproteins directed to the outer membrane are released from the inner membrane in an ATP-dependent manner through the formation of a complex with LolA, a periplasmic chaperone. However, the ATPase involved in this reaction has not been identified. Here we show, using reconstituted proteoliposomes, that a new complex, LolCDE, belonging to the ATP-binding cassette (ABC) transporter family, catalyses the release of lipoproteins in LolA- and sorting-signal-dependentmanners. The LolCDE complex differs mechanistically from all other ABC transporters as it is not involved in the transmembrane transport of substrates. This new mechanism is evolutionarily conserved in other Gram-negative bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:33:20