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Titolo:
Rearrangement of a stable RNA secondary structure during VS ribozyme catalysis
Autore:
Andersen, AA; Collins, RA;
Indirizzi:
Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 Genet, Toronto, ON M5S 1A8, Canada
Titolo Testata:
MOLECULAR CELL
fascicolo: 3, volume: 5, anno: 2000,
pagine: 469 - 478
SICI:
1097-2765(200003)5:3<469:ROASRS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELF-CLEAVAGE REACTION; GROUP-I INTRON; HAMMERHEAD RIBOZYME; CRYSTAL-STRUCTURE; RIBOSOMAL-RNA; SEQUENCE REQUIREMENTS; TERTIARY INTERACTIONS; MESSENGER-RNA; STEM-LOOP; NEUROSPORA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Collins, RA Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 to, ON M5S 1A8, Canada
Citazione:
A.A. Andersen e R.A. Collins, "Rearrangement of a stable RNA secondary structure during VS ribozyme catalysis", MOL CELL, 5(3), 2000, pp. 469-478

Abstract

The Neurospora VS ribozyme recognizes and cleaves a substrate RNA that contains a CC-rich stem loop. In contrast to most RNA secondary structures that are stable during tertiary or quaternary folding, this substrate undergoes extensive ribozyme-induced rearrangement in the presence of magnesium in which the base pairings of at least seven of the ten nucleotides in the stem are changed. This conformational switch is essential for catalytic activity with the wildtype substrate and creates a metal-binding secondary structure motif near the cleavage site. Base pair rearrangement is accompanied bybulging a cytosine from the middle of the stem, indicating that ribozymes may perform base flipping, an activity previously observed only with protein enzymes that modify DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:48:23