Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Gemfibrozil metabolite inhibits in vitro low-density lipoprotein (LDL) oxidation and diminishes cytotoxicity induced by oxidized LDL
Autore:
Kawamura, M; Miyazaki, S; Teramoto, T; Ashidate, K; Thoda, H; Ando, N; Kaneko, K;
Indirizzi:
Tokyo Teishin Hosp, Dept Internal Med, Div Endocrinol & Metab, Chiyoda Ku,Tokyo 102, Japan Tokyo Teishin Hosp Tokyo Japan 102 & Metab, Chiyoda Ku,Tokyo 102, Japan Teikyo Univ, Sch Med, Dept Med, Tokyo 173, Japan Teikyo Univ Tokyo Japan173 yo Univ, Sch Med, Dept Med, Tokyo 173, Japan Warner Lambert KK, Tokyo, Japan Warner Lambert KK Tokyo JapanWarner Lambert KK, Tokyo, Japan
Titolo Testata:
METABOLISM-CLINICAL AND EXPERIMENTAL
fascicolo: 4, volume: 49, anno: 2000,
pagine: 479 - 485
SICI:
0026-0495(200004)49:4<479:GMIIVL>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; HUMAN ATHEROSCLEROSIS; LIPID PEROXIDES; VITAMIN-E; IN-VITRO; ANTIOXIDANTS; MACROPHAGES; PROBUCOL; DISEASE; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kawamura, M Tokyo Teishin Hosp, Dept Internal Med, Div Endocrinol & Metab,Chiyoda Ku,2-14-23 Fujimi, Tokyo 102, Japan Tokyo Teishin Hosp 2-14-23 Fujimi Tokyo Japan 102 102, Japan
Citazione:
M. Kawamura et al., "Gemfibrozil metabolite inhibits in vitro low-density lipoprotein (LDL) oxidation and diminishes cytotoxicity induced by oxidized LDL", METABOLISM, 49(4), 2000, pp. 479-485

Abstract

We hypothesized that M1, a metabolite of gemfibrozil, may have antioxidantproperties because of its hydroxylated phenol ring, 5-(4-hydroxy-2,5-dimethyl-phenoxy)-2,2-dimethyl pentanoic acid. The susceptibility of low-densitylipoprotein (LDL) to oxidative modification was investigated by a method using 2,2-azobis(4-methoxy-2,4-dimethylvaleronitrile [MeO-AMVN]) or Cu2+ as previously reported. Conjugated dienes (CDs), lipid hydroperoxide (LPO), and thiobarbituric acid-reactive substances (TBARS) were measured to evaluatethe degree of LDL oxidation. Oxidized LDL (OxLDL), which is used for cytotoxicity studies, was prepared by the dialysis method using Cu2+ as the oxidation inducer. Cytotoxicity induced by OxLDL was studied in J774 macrophages by colorimetric assay using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT assay). The oxidative modification of LDL was inhibited by M1 in a dose-dependent manner. The antioxidant effect of M1 on LDL oxidation was diminished by dialysis of the LDL incubated with M1 against phosphate-buffered saline (PBS), suggesting that M1 is hydrophilic rather than lipophilic. M1 diminished the cytotoxicity induced by OxLDL, although it was milder versus probucol, These data suggest that this gemfibrozil metabolite has an antioxidant effect on LDL. and thus M1 may contribute to the antiatherogenic effects of gemfibrozil. Copyright (C) 2000 by W.B. Saunders Company.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:23:43