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Titolo:
Grass carp (Ctenopharyngodon idellus) bile may inhibit the release of renal dipeptidase from the proximal tubules by nitric oxide generation
Autore:
Choi, K; Park, SW; Lee, KJ; Lee, HB; Han, HJ; Park, SK; Park, HS;
Indirizzi:
Chonnam Natl Univ, Coll Pharm, Dept Pharm, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Coll Nat Sci, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Coll Vet Med, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonbuk Natl Univ, Coll Med, Chonju, South Korea Chonbuk Natl Univ Chonju South Korea niv, Coll Med, Chonju, South Korea
Titolo Testata:
KIDNEY & BLOOD PRESSURE RESEARCH
fascicolo: 2, volume: 23, anno: 2000,
pagine: 113 - 118
SICI:
1420-4096(2000)23:2<113:GC(IBM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELLS; INGESTION; FAILURE; RATS;
Keywords:
proximal tubules; carp bile; nitric oxide; renal dipeptidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Park, HS Chonnam Natl Univ, Coll Pharm, Dept Pharm, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
K. Choi et al., "Grass carp (Ctenopharyngodon idellus) bile may inhibit the release of renal dipeptidase from the proximal tubules by nitric oxide generation", KIDNEY BL P, 23(2), 2000, pp. 113-118

Abstract

There are many reports on acute renal failure (ARF) after ingestion of grass carp bile (CB; Ctenopharyngodon idellus). Renal dipeptidase (RDPase; EC 3.4.13.19) is a glycosylphosphatidylinositol-anchored ectoenzyme within therenal proximal tubules (PTs) and is proposed as a diagnostic enzyme of renal disease. We examined the release of RDPase following treatment with CB and various nitric oxide (NO) related compounds in porcine PTs. The RDPase release from PTs was inhibited by CB in a concentration-dependent manner andwas also inhibited by sodium nitroprusside (direct NO donor) and L-arginine (NO synthase substrate) in the tested range (0-12 mM). CB-treated (0.1 mg/ml) PTs showed a decreased RDPase activity in comparison with the control group. This inhibition was blocked by 2 mM L-NAME (NO synthase inhibitor) and U73122 (inhibitor of phosphatidylinositol-specific phospholipase C) in aconcentration-dependent manner. Eel bile (0-0.1 mg/ml), used as the control, did not significantly affect the RDPase release from PTs. The NO concentration was observed as nitrite, the degradation product of the NO metabolism, increased in proportion to CB and L-arginine. The increase of nitrite to151.5% by CB treatment (0.1 mg/ml) was blocked by 2 mM L-NAME (95.5%). When the phospholipase C pathway was blocked by 10 and 20 mu M U73122, the nitrite generation decreased to 122.7 and 89.4%, respectively. These results strongly suggest that NO generation and the phospholipase C pathway affect the RDPase release from the PTs and that they may be involved in the development of ARF in vivo following CB ingestion. The release of RDPase from PTs could be a useful tool not only for this CB-caused ARF, but also for the elucidation of other biochemical mechanisms. Copyright (C) 2000 S. Karser AG,Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 23:46:35