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Titolo:
The use of chimeric venezuelan equine encephalitis viruses as an approach for the molecular identification of natural virulence determinants
Autore:
Powers, AM; Brault, AC; Kinney, RM; Weaver, SC;
Indirizzi:
Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 h, Dept Pathol, Galveston, TX 77555 USA Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 h, Dept Pathol, Galveston, TX 77555 USA Ctr Dis Control, Ctr Infect Dis, Div Vector Borne Infect Dis, US Dept HHS,Publ Hlth Serv, Ft Collins, CO 80522 USA Ctr Dis Control Ft Collins CO USA80522 th Serv, Ft Collins, CO 80522 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 9, volume: 74, anno: 2000,
pagine: 4258 - 4263
SICI:
0022-538X(200005)74:9<4258:TUOCVE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOSQUITO CELLS; SOUTH-AMERICA; STRAIN TC-83; SINGLE; REPLICATION; MUTATIONS; REGION; GLYCOPROTEINS; ATTENUATION; NUCLEOTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Powers, AM Univ Texas, Med Branch, Dept Pathol, 301 Univ Blvd, Galveston, TX 77555 USA Univ Texas 301 Univ Blvd Galveston TX USA 77555 n, TX 77555 USA
Citazione:
A.M. Powers et al., "The use of chimeric venezuelan equine encephalitis viruses as an approach for the molecular identification of natural virulence determinants", J VIROLOGY, 74(9), 2000, pp. 4258-4263

Abstract

Venezuelan equine encephalitis (VEE) virus antigenic subtypes and varieties are considered either epidemic/ epizootic or enzootic. In addition to epidemiological differences between the epidemic and enzootic viruses, severalin vitro and in vivo laboratory markers distinguishing the viruses have been identified, including differential plaque size, sensitivity to interferon (IFN), and virulence for guinea pigs, These observations have been shown to be useful predictors of natural, equine virulence and epizootic potential. Chimeric viruses containing variety LAB (epizootic) nonstructural genes with variety IE (enzootic) structural genes (VE/IAB-IE) or IE nonstructuralgenes and IAB structural genes (IE/IAB) were constructed to systematicallyanalyze and map viral phenotype and virulence determinants. Plaque size analysis showed that both chimeric viruses produced a mean plaque diameter that was intermediate between those of the parental strains. Additionally, both chimeric viruses showed intermediate levels of virus replication and virulence for guinea pigs compared to the parental strains. However, IE/IAB produced a slightly higher viremia and an average survival time 2 days shorter than the VE/IAB-IE virus. Finally, IFN sensitivity assays revealed that only one chimera, VE/IAB-IE, was intermediate between the two parental types. The second chimera, containing the IE nonstructural genes, was at least five times more sensitive to IFN than the IE parental virus and greater than50 times more sensitive than the IAB parent, These results implicate viralcomponents in both the structural and nonstructural portions of the genomein contributing to the epizootic phenotype and indicate the potential for epidemic emergence from the IE enzootic VEE viruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 06:42:45